- 期刊
- OpenAccess
Major Viral Diseases of Penaeus Monodon in Taiwan
臺灣草蝦主要病毒癥之研究回顧與前瞻
摘要
本文敘述為因應九孔大量人工受精與染色體操作之目的而研發之一套初級自動化系統。該系統係根據操作流程中模擬試驗所表現之最佳各項條件所設計。試驗發現九孔卵在未受精前頗能耐受暴露於水或空氣中，可分別長達210分及80分。受精卵堆疊的高度在0.8公分連續60分鐘不至受損。受精卵在長達4 m之輸送管道中未見外觀傷害或受精率下降。但受精卵在濃縮機150，200，250 rpm轉速20分時受精率明顯下降。使用此次設計之自動化系統建議轉速在40 rpm；且馬達抽水率在20.0±3 liter／min。受精卵在濃縮槽內之輪鼓旋轉攪拌下有益於孵化率之提昇，整個自動化系統的最終設計符合上述之各項特性。至於自動控制箱具有確認大量人工受精與染色體操作時之各項階段性處理能確實依序準時完成的功能。綜合言之，該系統用於九孔大量人工受精與染色體操作甚具可行性且係因應上述目的之自動化系統之首創。
並列摘要
Preliminary results are reported on an automatic manipulation system for the masspropagation and chromosome manipulation of the small abalone,Haliotis diversicolor.The system was designed based on the optimum conditions obtained from tests simulatingthe possible conditions during automatic manipulation.Small abalone eggs couldwithstand seawater and air exposure for up to 210 min and 80 min,respectively,beforefertilization.Fertilized eggs could accumulate up to 0.8 cm thick for 60 min withoutdamage.Transfer of the fertilized eggs through outlet pipes up to 4 m in length did notresult in visible damage or decline in hatching rate.Fertilized eggs were not resistant tocontinuous rotation at 150,200,or 250 rpm for 20 min.The rotating drum speed of theautomatic manipulation system within 40 rpm and the pump flow at 30±3 liter/min wererecommended.Effect of aeration and stirring of the rotating drum on the fertilized eggswithin the concentration tank improved hatching rates.The final design of the automaticmanipulation system incorporated these features.The automatic control box of thesystem ensures that specific treatments,such as those for chromosome manipulation,canbe precisely carried out.As a whole,the system has been found highly applicable forregular mass propagation and chromosome manipulation procedures of the small abaloneand is the first one of its kind.