Susceptibility of Penaeus japonicus, P. monodon, and P. semisulcatus to White Spot Syndrome Virus (WSSV)

白點症病毒可感染養殖及野生的蝦類、蟹類以及其他節肢動物。在本研究中，我們利用浸泡及餵食等兩種入工感染方式使斑節蝦第28期後幼蟲(PL28)感染白點症病毒。感染15天後，浸泡組與餵食組的死亡率分別到達88%與94%。檢測浸泡組存活的三個樣本，發現皆已受到白點症病毒感染，但是僅有一個為一階PCR正反應；檢測餵食組存活的三個樣本，僅兩個受到白點症病毒感染，其中只有一個為一階PCR正反應。然而無論是浸泡組或餵食組中，所有的垂死樣本皆為一階PCR正反應。我們進一步以浸泡感染的方式進行草蝦、斑節蝦以及熊蝦的感受性實驗，感染15天後，斑節蝦與草蝦的累積死亡率皆超過80%，而熊蝦僅為26%到47%。斑節蝦與草蝦的實驗組中，幾乎所有的垂死樣本皆為一階PCR正反應；但是熊蝦的實驗組中，並無一階PCR正反應的樣本。因此，我們推論熊蝦對白點症病毒的感受性較草蝦和斑節蝦低。

關鍵字

白點症病毒感染；蝦類感受性；聚合鏈反應

並列摘要

White spot syndrome virus (WSSV) has been detected in cultured and captured shrimps, crabs, pest crabs and other arthopods. In this study, Penaeus japonicus postlarvae (PL28) were experimentally challenged by WSSV via two transmission pathways: immersion and feeding. By 15 days post-challenge, mortality in the immersion and feeding groups reached 88% to 94%. Challenge by immersion produced an earlier onset of mortality than challenge by feeding. Only 1 out of 3 of the tested surviving shrimp from the immersion group was one-step PCR positive, but all 3 tested shrimp were WSSV infected. For the feeding group, 1 out of 3 tested survivors was one-step PCR positive and 2 out of 3 were WSSV infected. All the moribund specimens f or the immersion and feeding groups were one-step PCR positive. The susceptibility of P. monodon, P. japonicus and P. semisulcatus to WSSV was also determined by immersion challenge test. After 15 days, cumulative mortality reached more than 80% in P. japonicus and P. monodon, but only 26%-47% in P. semisulcatus. Almost all of the tested moribund P. japonicus and P. monodon samples were one-step WSSV PCR positive. None of the P. semisulcatus were one-step PCR positive. We concluded that P. semisulcatus is less susceptible than P. japonicus and P. monodon to WSSV infection.

並列關鍵字

WSSV infection ； Susceptibility of shrimp ； PCR

國際替代計量

Susceptibility of Penaeus japonicus, P. monodon, and P. semisulcatus to White Spot Syndrome Virus (WSSV)

全文下載

主題瀏覽