- 期刊
- OpenAccess
Requirements of Dietary Myo-inositol of Juvenile Grouper, Epinephelus malabaricus
石斑魚稚魚之肌醇需求量
摘要
An eight-week growth experiment was conducted to estimate dietary myo-inositol (MI) requirements for juvenile grouper, Epinephelus malabaricus. MI was supplemented at 0, 150, 250, 350, 450, 600 and 1,200mg/kg diet in the basal diet providing the actual values of 0, 145, 243, 362, 476, 622 and 1,181mg MI/kg diet, respectively. Basal diet without MI but with succinylsulfathiazole to suppress MI synthesis by intestinal bacteria was included for comparison. Each diet was fed to triplicate groups of grouper (mean initial weight 6.02±0.08g, n=3). Fish fed diets supplemented with ≥362mg MI/kg had highest (P＜0.05) weight gain, followed by fish fed diets with 145 and 243mg MI/kg and lowest in fish fed the unsupplemented control diets. Fish fed diets with ≥145mg MI/kg diet had higher feed efficiency than fish fed the unsupplemented control diets. Supplementation of dietary inositol did not affect survival of the grouper. The myo-inositol concentration in liver was highest in fish fed ≥243mg MI/kg diets, followed by fish fed 145mg MI/kg diet and lowest in fish fed the unsupplemented control diets. Hepatic lipid concentration was lower in fish fed diets with ≥145mg MI/kg diet than fish fed the unsupplemented control diets. Weight gain percentage and the hepatic myo-inositol concentration for the different treatments were analyzed by broken-line regression and indicated that the requirement for dietary myo-inositol in growing grouper is about 335-365mg/kg diet. Addition of an antibiotic to basal diet did not affect the growth and hepatic inositol concentration of the fish, suggesting that the intestinal microbial synthesis was not a significant source of inositol for grouper.
並列摘要
An eight-week growth experiment was conducted to estimate dietary myo-inositol (MI) requirements for juvenile grouper, Epinephelus malabaricus. MI was supplemented at 0, 150, 250, 350, 450, 600 and 1,200mg/kg diet in the basal diet providing the actual values of 0, 145, 243, 362, 476, 622 and 1,181mg MI/kg diet, respectively. Basal diet without MI but with succinylsulfathiazole to suppress MI synthesis by intestinal bacteria was included for comparison. Each diet was fed to triplicate groups of grouper (mean initial weight 6.02±0.08g, n=3). Fish fed diets supplemented with ≥362mg MI/kg had highest (P＜0.05) weight gain, followed by fish fed diets with 145 and 243mg MI/kg and lowest in fish fed the unsupplemented control diets. Fish fed diets with ≥145mg MI/kg diet had higher feed efficiency than fish fed the unsupplemented control diets. Supplementation of dietary inositol did not affect survival of the grouper. The myo-inositol concentration in liver was highest in fish fed ≥243mg MI/kg diets, followed by fish fed 145mg MI/kg diet and lowest in fish fed the unsupplemented control diets. Hepatic lipid concentration was lower in fish fed diets with ≥145mg MI/kg diet than fish fed the unsupplemented control diets. Weight gain percentage and the hepatic myo-inositol concentration for the different treatments were analyzed by broken-line regression and indicated that the requirement for dietary myo-inositol in growing grouper is about 335-365mg/kg diet. Addition of an antibiotic to basal diet did not affect the growth and hepatic inositol concentration of the fish, suggesting that the intestinal microbial synthesis was not a significant source of inositol for grouper.