A Vaccination Trial Using Attenuated Photobacterium damselae ssp. piscicida in Cobia

海鱺巴斯德桿菌減毒疫苗免疫試驗

古鎮鈞(Chen-Chun Ku) ； 王俊順(Chun-Shun Wang) ； 陸知慧(Chih-Hui Lu)

《臺灣水產學會刊》 35卷3期 (2008/09) Pp. 281-288

https://doi.org/10.29822/JFST.200809.0007

摘要

本試驗將Photobacterium damselae ssp. piscicida以連續培養方式製備成減毒疫苗，並以浸泡法免疫海鱺魚苗。實驗室條件下進行攻毒實驗,其相對保護率(RPS)為55.7%。將存活下來的海鱺進行第二次攻毒實驗，其相對保護率則高達83.3%及91.6%。然而在海水箱網養殖場的環境下，疫苗組的死亡率卻達79.3%，甚至比對照組的77.3%還高。現場實驗存活下的海鱺自此也不再感染巴斯德桿菌症。檢驗擬集抗體在非疫苗組，疫苗組及攻擊試驗後存活下來的海鱺血液內的變化，發現海鱺對抗巴斯德桿菌的能力和其體內擬集抗體的力價高低無關。

關鍵字

海鱺 ； 巴斯德桿菌 ； 減毒疫苗 ； 感染後免疫

並列摘要

Attenuated bacterin was prepared from Photobacterium damselae subsp. piscicida (Ph. d. p.) against cobia pasteurellosis by immersion vaccination. In the laboratory aquarium challenge, the vaccine gave a relative percentage survival (RPS) of 55.7% after challenge with Ph. d. p. Moreover, these laboratory aquarium challenge survivors got RPS values of 83.3% and 91.6% after receiving a second challenge with Ph. d. p. But in farm trial, the mortality rate was 79.3% for the vaccine group and was even higher than the control group (77.3%). These field trial survivors also never developed pasteurellosis again during the sea cage culture period. The variation among the agglutinating antibody titers in non immunized, immunized and survivors cobia was irrelevant to the protection against Ph. d. p.

並列關鍵字

cobia ； pasteurellosis ； attenuated vaccine ； post-infectious immunity