Study on Ethanol Fermentation from Monostroma nitidum Hydrolysate Solution

以青海菜（Monostroma nitidum）水解液發酵酒精之研究

陳君如(Jiun-Ru Chen) ； 潘崇良(Chorng-Liang Pan) ； 曾國政(Kuo-Chang Zen) ； 吳紹祺(Shao-Chi Wu)

《臺灣水產學會刊》 36卷3期 (2009/09) Pp. 191-203

https://doi.org/10.29822/JFST.200909.0003

摘要

One percent (w/v) Monostroma (M.) nitidum solution was hydrolyzed using 1 unit/ml five enzymes MA103-agarases, MAEF108-agarases, α-amylase, β-galactosidase, or cellulase, respectively, at 40℃ for 24 h. The reducing sugar content of the 1% (w/v) M. nitidum solutions all reached the stationary phase after enzymatic digestion for 6 h. MAEF108-agarases and cellulase showed to have a better reducing sugar content than the rest. When 0.5 to 3.0% M. nitidum solution were digested using 10 units/ml MAEF108-agarases and 10 units/ml cellulase, the reducing sugar contents (3.97-6.56 mg/ml) greater than 2.0% had no significant difference (p ＜ 0.05). Ten Saccharomyces (S.) cerevisiae were tested for fermented ethanol from a M. nitidum hydrolysate solution, and four S. cerevisiae: BCRC20581, BCRC21686, BCRC21824, and BCRC21962 had the highest ethanol content (5.91, 6.30, 6.57, and 6.18%, respectively) among them. Next, the above four strains were arranged in pairs for ethanol producing starter groups; S5: S. cerevisiae BCRC21686 and BCRC21962, S6: S. cerevisiae BCRC21824 and BCRC21962 had the highest ethanol content (6.94 and 6.87%) of the six groups. As a result, the ethanol producing starter groups S5 and S6 as described in this study should be used in the future for making ethanol from a M. nitidum hydrolysate solution.

關鍵字

洋菜酶 ； 纖維素酶 ； 乙醇 ； 青海菜

並列摘要

One percent (w/v) Monostroma (M.) nitidum solution was hydrolyzed using 1 unit/ml five enzymes MA103-agarases, MAEF108-agarases, α-amylase, β-galactosidase, or cellulase, respectively, at 40℃ for 24 h. The reducing sugar content of the 1% (w/v) M. nitidum solutions all reached the stationary phase after enzymatic digestion for 6 h. MAEF108-agarases and cellulase showed to have a better reducing sugar content than the rest. When 0.5 to 3.0% M. nitidum solution were digested using 10 units/ml MAEF108-agarases and 10 units/ml cellulase, the reducing sugar contents (3.97-6.56 mg/ml) greater than 2.0% had no significant difference (p ＜ 0.05). Ten Saccharomyces (S.) cerevisiae were tested for fermented ethanol from a M. nitidum hydrolysate solution, and four S. cerevisiae: BCRC20581, BCRC21686, BCRC21824, and BCRC21962 had the highest ethanol content (5.91, 6.30, 6.57, and 6.18%, respectively) among them. Next, the above four strains were arranged in pairs for ethanol producing starter groups; S5: S. cerevisiae BCRC21686 and BCRC21962, S6: S. cerevisiae BCRC21824 and BCRC21962 had the highest ethanol content (6.94 and 6.87%) of the six groups. As a result, the ethanol producing starter groups S5 and S6 as described in this study should be used in the future for making ethanol from a M. nitidum hydrolysate solution.

並列關鍵字

Agarase ； Cellulase ； Ethanol ； Monostroma nitidum