Antivirus and Prebiotic Properties of Seaweed-oligosaccharide-lysates Derived from Agarase AS-II

經Agarase AS-II水解所得海藻寡醣之抗病毒及益生特性

吳紹祺(Shao-Chi Wu) ； 康新楷(Sheen-Kye Kang) ； 卡伯特(Bartosz Kazlowski) ； 吳彰哲(Chang-Jer Wu) ； 潘崇良(Chorng-Liang Pan)

《臺灣水產學會刊》 39卷1期 (2012/03) Pp. 11-21

https://doi.org/10.29822/JFST.201203.0002

摘要

Gra-C108、Mon-C108、Gra-AS-II、或Mon-AS-II為龍鬚菜萃取多醣（Gra）或海菜萃取多醣（Mon）經A. salmonicida MAEF108粗酵素液或Agarase AS-II降解所得海藻寡醣。不同濃度的Gra、Mon、Gra-C108、Mon-C108、G-AS-II、或Mon-AS-II海藻樣品與不同濃度的BHK-21細胞進行培養，發現皆不具細胞毒性，且細胞存活率皆高於78%。6種藻類樣品均呈現降低日本腦炎病毒北京一型感染BHK-21細胞的現象，且硫酸根含量較高之海菜樣品降低感染的能力優於龍鬚菜樣品。三株雙歧桿菌以6種藻類樣品做為生長碳源以評估益生質能力。當Bifidobacterium （B.） pseudolongum subsp. pseudolongum BCRC14673利用Gra-C108時，24小時之內活菌數可以增加到超過3 log CFU/ml。海菜萃取多醣及其水解物亦皆能夠做為雙歧桿菌生長利用的碳源。B. adolescentis BCRC 14608以及B.pseudolongum subsp. pseudolongum BCRC 14673皆可利用上述6種藻類樣品且產酸降低pH值。B. longum BCRC 11847較能利用龍鬚菜樣品產酸降低pH值。

關鍵字

β-洋菜酶 ； 抗病毒 ； 益生性

並列摘要

The seaweed-oligosaccharide-lysates (SwOSLys) Gra-C108, Mon-C108, Gra-AS-II, or Mon-AS-II are seaweed polysaccharide extracts (SwPSExts) Gra (Gracilaria sp.) or Mon (Monostroma nitidum), as digested by either crude MAEF108-agarases or Agarase AS-II produced from marine bacteria Aeromonas salmonicida MAEF108. Different concentrations of Gra, Mon, Gra-C108, Mon-C108, Gra-AS-II, or Mon-AS-II were cultured with different cell densities of BHK-21 kidney cells, in which the SwPSExts or SwOSLys samples showed no cytotoxicity, and the cell viability was higher than 78%. The six seaweed samples displayed an ability to decrease the effects caused by Japanese encephalitis virus (JEV, Beijing-1 strain) infection. The six seaweed samples were tested as a carbon source for bifidobacteria growth to evaluate the prebiotic potentials. Viable bacteria counts of Bifidobacterium (B.) pseudolongum subsp. pseudolongum BCRC 14673 could increase more than 3 log CFU/ml in 24 h incubation, when Gra-C108 is utilized. Mon and its agarase lysates can be used by bifidobacteria as a carbon source. B. adolescentis BCRC 14608 and B. pseudolongum subsp. pseudolongum BCRC 14673 could utilize the six seaweed samples to lower pH. B. longum BCRC 11847 displayed an increased pH-lowering ability when utilizing the Gracilaria sp. (red algae) than Mon. nitidum (green algae) as based samples.

並列關鍵字

β-agarase ； Antivirus ； Prebiotic