A feeding trial was conducted to investigate the dietary zinc (Zn) requirements of soft-shelled turtles fed diets containing sodium phytate. Juvenile soft-shelled turtles were fed diets containing 7 levels of Zn (13, 40, 66, 91, 114, 147, and 177 mg kg^(-1)) for 8 weeks. Each diet was also supplemented with 12 g kg^(-1) sodium phytate. Turtles fed the control diet containing 13 mg kg^(-1) endogenous Zn showed lower weight gain (WG), feed conversion ratio (FCR) and protein efficiency ratio (PER) than those fed diets supplemented with Zn (p≤0.05). The Zn contents in liver, serum, and carapaces of the turtles fed low Zn diets (13 and 40 mg kg^(-1)) were lower (p≤0.05) than other groups. In the presence of 1% phytic acid, dietary Zn requirements of juvenile soft-shelled turtles derived from regression modeling with liver, serum and carapace Zn contents as the dependent variables were 157, 148 and 154 mg Zn kg^(-1), respectively. This value is approximately 3 folds of the requirement that has been reported for soft-shelled turtles when phytic acid-free diets were given to the turtles.