Growth Inhibition of Carcinoma Cells by Sodium Alginate from Petalonia Binghamiae (Phaeophyceae)

海藻Petalonia binghamiae褐藻酸鈉抑制癌細胞生長的活性

李惠婷(Hui-Ting Lee) ； 黃穰(Rang Huang)

《臺灣水產學會刊》 39卷4期 (2012/12) Pp. 257-267

https://doi.org/10.29822/JFST.201212.0005

摘要

褐藻酸鈉是褐藻的重要內含物及結構成分，且有多種生物活性。此多糖對二價金屬離子有很強的親和力因而導致分子構形易改變。根據本研究結果，從海藻Petalonia binghamiae萃取出的褐藻酸鈉能抑制人類胰臟癌及乳癌細胞的增殖。惟該多糖的抑癌活性在經過檸檬酸水解後顯著降低，而遇到鈣，鋅，鈷，錳等二價離子則會失去抑制活性。但，這些金屬離子若經過Na2EDTA的前處理就不會影響褐藻酸鈉的抑癌活性。上述結果顯示在褐藻酸羥基上的離子交換情形以及分子構形是影響該多糖抑制癌細胞反應的重要因素。再者，褐藻酸鈉能促進老鼠巨噬細胞增殖及分泌蛋白質類物質。分泌的蛋白質能顯著抑制癌細胞的生長。由於褐藻酸鈉廣泛而又多量地分佈在褐藻。因此，該多糖在抑制癌細胞的研究及應用上極具有潛力。

關鍵字

褐藻酸鈉 ； 癌細胞 ； 抑制活性 ； 巨噬細胞液 ； 檸檬酸緩衝液 ； 金屬離子

並列摘要

Alginate is an important structural component of brown algae and has various biological activities. In addition, the polymer has high affinity for binding divalent metal ions, leading to change in molecular conformation. In the present study, sodium alginate extracted from the brown alga Petalonia binghamiae inhibited markedly the proliferation in human pancreatic and breast adenocarcinoma cells. The inhibitory activity of alginate decreased after being treated with citrate buffer solution and lost in the presence of Ca^2+, Zn^2+, Co^2+ or Mn^2+. However, alginate remained high inhibitory activity if metal ions were treated with Na2EDTA prior to addition to culture medium. These results suggested that ion exchange at carboxyl groups as well as molecule conformation of the polymer played an important role in alginate inhibitory reaction with carcinoma cells. Notably, the obtained alginate induced murine macrophages to produce proteinaeous substance that significantly inhibited carcinoma cell proliferation. Our study suggests that sodium alginate has potent use in anticancer therapy and research due to its wide distribution and high production in marine brown algae.

並列關鍵字

Sodium alginate ； Carcinoma cells ； Inhibitory activity ； Macrophage supernatant ； Citrate buffer solution ； Metal ions