Dietary Sargassum glaucescens Acidic-aqueous Extract Administered to Enhance Immune Responses and Disease Resistance of Tiger Shrimp Penaeus monodon

飼料添加粉葉馬尾藻酸性溶液萃取物增強草蝦免疫反應及抗病能力

張欽泉(Chin-Chyuan Chang) ； Franklin Saúl Martínez Toscano ； 鄭文騰(Winton Cheng)

《臺灣水產學會刊》 40卷1期 (2013/03) Pp. 57-70

https://doi.org/10.29822/JFST.201303_40(1).0005

摘要

The immune parameters and the resistance against Photobacterium damselae of tiger shrimp Penaeus monodon (14.2 ± 1.8 g) were evaluated when the shrimp were fed diets containing the acidicaqueous extract of Sargassum glaucescens (SGE) at 0 (control), 0.5, and 1.0 g/kg for 21 days. Results showed that P. monodon fed a diet containing 1.0 g/kg SGE had significantly increased phenoloxidase activity and respiratory bursts, but decreased superoxide dismutase activity (p ＜ 0.05). The glutathione peroxidase and transglutaminase activities, clotting rate, and phagocytic activity and clearance efficiency toward P. damseale were significantly elevated in shrimp fed 0.5 and 1.0 g/kg SGE-containing diets compared to control shrimp (p ＜ 0.05). In challenged test, the survival of shrimp fed a diet containing 1.0 g/kg SGE was significantly higher (p ＜ 0.05) than that of control shrimp after 7 days. We therefore recommend dietary SGE administration at 1.0 g/kg to enhance the immunity and resistance against P. damselae in P. monodon.

關鍵字

飼料添加 ； 草蝦 ； 粉葉馬尾藻萃取物 ； 免疫反應 ； 抗病能力

並列摘要

The immune parameters and the resistance against Photobacterium damselae of tiger shrimp Penaeus monodon (14.2 ± 1.8 g) were evaluated when the shrimp were fed diets containing the acidicaqueous extract of Sargassum glaucescens (SGE) at 0 (control), 0.5, and 1.0 g/kg for 21 days. Results showed that P. monodon fed a diet containing 1.0 g/kg SGE had significantly increased phenoloxidase activity and respiratory bursts, but decreased superoxide dismutase activity (p ＜ 0.05). The glutathione peroxidase and transglutaminase activities, clotting rate, and phagocytic activity and clearance efficiency toward P. damseale were significantly elevated in shrimp fed 0.5 and 1.0 g/kg SGE-containing diets compared to control shrimp (p ＜ 0.05). In challenged test, the survival of shrimp fed a diet containing 1.0 g/kg SGE was significantly higher (p ＜ 0.05) than that of control shrimp after 7 days. We therefore recommend dietary SGE administration at 1.0 g/kg to enhance the immunity and resistance against P. damselae in P. monodon.

並列關鍵字

Dietary administration ； Penaeus monodon ； Sargassum glaucescens extracts ； immune responses ； disease resistance