蚯蚓腔腸之染料脫色菌株生產生物可分解高分子材料之可行性研究

A Feasibility Study of Using Dye-Decolorizing Microbes from Earthworm Guts for Biodegradable Polymer Polyhydroxyalkanoate Production

蕭子昭(T.J. Shiau) ； 余秉樺(B.H. Yu) ； 陳博彥(B.Y. Chen) ； 魏毓宏(Y.H. Wei)

《鑛冶：中國鑛冶工程學會會刊》 55卷3期 (2011/09) Pp. 93-103

https://doi.org/10.30069/MM.201109.0009

摘要

本研究自台灣本土紅蚯蚓腔腸內篩選具功能性微生物以作為綠色生物材料生產之應用以探討染整廢水處理同時生產生物可分解性高分子（聚羥基烷酯類, PHA）之可行性。首先將蚯蚓經濾紙法排土後，取液體以平板塗抹出具脫色活性之菌落，再以外加其他營養源之PBS培養基培養下，分別以14種不同（非）偶氮染料環境下進行初步篩選，選擇脫色能力較佳之2種染料作為後續污染物能力馴化之篩選組合，進行連續式馴化並加以比較其污染物處理能力。同時將所選擇之組合經以月桂酸作為碳源條件進行批次培養，以含有較高之PHA含量及濃度的組合進行限制養分之連續馴化。再與先前研究自行自東北及西南台灣篩選之嗜水性產氣單胞菌NIU01及YTl1分別以月桂酸為最佳碳源培養之PHA產量作為評比，以分析未來開發應用於染整廢水處理並同時回收生物可分解性材料之可行性。

關鍵字

資源回收再利用 ； 染料脫色 ； 生物可分解性高分子 ； Eisenia fetida

並列摘要

This study tended to isolate functioning microorganisms from guts of indigenous earthworm Eisenia fetida for producing green biopolymer materials and explore the feasibility of materials recycling for biodegradable polymer polyhydroxyalkanoates (PHAs) production. Excrements of earthworm were first harvested via the filter paper method and then dye-decolorizing consortia were isolated via a serial streaking isolation of agar-plating. Preliminary screening for dye-decolorizing consortia with higher decolorizing capabilities was conducted using glycerol-containing phosphate-buffered saline (PBS) media supplemented with diverse dyes. Two probing dyes with best color removal performance for feasibility study. A serial lauric acid-bearing batch cultures of various combination of consortia were conducted in nutrientlimiting conditions to evaluate the efficiencies of PHA production. Our findings were compared with that of Aeromonas hydrophila NIU01 and YTl1 to assess the feasibility of these selected strains for producing biopolymer PHA.