以蝴蝶蘭白化葉片誘導擬原球體與植株再生

Plant Regeneration from Protocorm-like Bodies Induced in Etiolated Leaves of Phalaenopsis aphrodite Rchb. F.

許家嘉(Chia-Chia Hsu) ； 陳福旗(Fure-Chyi Chen)

《中國園藝》 49卷4期 (2003/12) Pp. 335-341

https://doi.org/10.6964/JCSHS.200312.0335

摘要

本試驗以蝴蝶蘭Phal. aphrodite Rchb. f.之實生苗，經黑暗處理所獲得之白化葉片來誘導擬原球體及植株再生，以建立有效率之無性繁殖體系。葉片分化程度、蔗糖濃度與生長調節劑爲影響擬原球體發生之主要因子，白化葉片具有幼嫩細胞，比綠色葉片逆分化能力強且容易產生擬原球體。誘導擬原球體最佳的蔗糖濃度爲0.3%，高濃度之蔗糖不利於擬原球體發生與增殖，且易導致培植體褐化。誘導擬原球體發生與增殖最佳的生長調節劑濃度爲0.5mg/l BA與0.5mg/l TDZ組合，BA與較高濃度2,4-D組合會抑制擬原球體發生與增殖。將擬原球體繼代於不含生長調節劑之培養基中可以持續產生擬原球體。由葉片誘導之擬原球體移植至再生培養基，照光培養3週，可獲得再生植株，移植至溫室生長良好。

關鍵字

蝴蝶蘭 ； 擬原球體 ； 再生

並列摘要

Etiolated leaves of in vitro seedlings from Phalaenopsis. aphrodite Rchb. f. grown in the dark were used as explants to induce PLBS and subsequent plant regeneration for efficient vegetative propagation. The developmental stage of leaves, sucrose concentration and plant growth regulator are main factors effecting PLBS formation. The etiolated leaves are much easier to dedifferentiate into PLBS than green leaves. The optimal concentration of sugar for PLB formation is 0.3%. High concentration of sucrose was inhibitory to the formation and proliferation of PLBS since explants turned browning easily. Explants on medium supplemented with 0.5mg/l BA and 0.5mg/l TDZ produced a large number and proliferation of PLBS. BA combined with higher level 2, 4-D inhibited PLBS formation and proliferation. PLBS could multiply further on growth regulator free medium. Development of normal plantlets was obtained after transferring PLBS onto regeneration medium for three weeks under light and subsequent transfer to greenhouse.

並列關鍵字

Phalaenopsis ； Protocorm-like bodies PLBs ； Regeneration