銀葉桉組織培養之建立

The Establishment of Eucalyptus cinerea Muell. ex Benth. in vitro

李秋梅(Chiu-Mei Li) ； 黃敏展(Min-Chang Huang)

《中國園藝》 49卷4期 (2003/12) Pp. 353-360

https://doi.org/10.6964/JCSHS.200312.0353

摘要

本研究建立銀葉桉(Eucalyptus cinerea F. Muell. ex Benth.)矮性實生變異株之初代培養。取一年生銀葉桉之單節培殖體，以1%的NAOCL消毒10分鐘後培養可獲得最高的萌芽率。培養基中加入1g/l聚乙烯比咯酮(Polyvinylpyrrolidone, PVP-40T)抑制褐化效果最好。培殖體以在頂端10cm的腋芽萌芽率最高，並以夏季七月所取的褐化情形最少，污染率最低且萌芽率最高。銀葉桉初代培養基以MS (Murashige and Skoog, 1962)全量、0.1mg/l BA、0.01mg/lNAA、1g/lPVP-40T、蔗糖30g/l、洋菜8g/l、pH值5.7的配方最佳。

關鍵字

銀葉桉 ； 節培殖體 ； 褐化 ； 植物生長調節劑

並列摘要

In this experiment, the initial culture of Eucalyptus cinerea 'Mini' was established. The sprouting rate of one-node explants from one year old stem which was disinfested by dipping in 1% NAOCL for 10 min. The medium with 1g/l polyvinylpyrrolidone inhibited the browning of explants more efficiently. The sprouting rate of lateral shoot of one-node cuttings from the upper 10cm of the stem was the highest. In addition, explants harvested in July resulted in the least browning and contamination. Moreover, the best medium was MS basal medium containing 0.1mg/lBA, 0.01mg/lNAA, 1g/lPVP-40T, 30g/l sucrose and 8g/l agar, and pH at 5.7.

並列關鍵字

Eucalyptus cinerea ； nodal explants ； browning ； plant regulators