松杉靈芝之栽培及其菌絲之深層培養

Cultivation of Ganoderma tsugae and Its Mycelium in Submerged Culture

曾裕琇(Yu-Hsiu Tseng) ； 蔡淑瑤(Shu-Yao Tsai) ； 李煜玲(Yu-Ling Lee) ； 毛正倫(Jeng-Leun Mau)

《農林學報》 52卷3期 (2003/09) Pp. 35-47

https://doi.org/10.30089/JAF.200309.0003

摘要

The research reported herein was to study diverse production methods of Ling chih (Ganoderma tsugae Murrill). In polypropylene, bag cultivation, Ling chih was suitably inoculated in March in Mid-Taiwan region, and harvested during May to November. Ling chili was harvested every 20 days and each bag produced about 75g of Ling chih and about S fruiting bodies, while baby Ling chih was harvested every 10 days and each bag produced about 101 g of baby Ling chih and about 9 fruiting bodies. With regards to the biological efficiency in fruiting body cultivation, baby Ling chih was evidently higher than Ling chih (1.05＞0.56). In shaken flask culture, the relationship of dry mycelial biomass and pH value, residual sugar content, laccase activity and polysaccharide concentration indicated that at day 12 the highest amount of dry biomass (18.74 g/L) was obtained with the highest amount polysaccharide concentration (1.38 g/L). The results of proximate composition showed that the carbohydrate, ash, crude lipid and crude protein contents of mycelia were 26.98, 5.18, 21.86 and 26.59%, respectively, significantly higher than those of fruiting bodies (both Ling chih and baby Ling chih), which were 10.41-17.17, l.69-2.62, 5.72-6.50 and 8.81-13.78%, respectively. In terms of crude fiber contents, Ling chih (73.37%) obviously more than baby Ling chih (59.93%), and in turn exceedingly higher mycelia (19.39%) and fermentation filtrate (1.58%). This research could provide valuable information for cultivation and submerged culture of native Ling chih. Also, the physiological effects of Ling chili, baby Ling chih, mycelia and fermentation filtrate could be a further area of investigation.

關鍵字

松杉靈芝 ； 靈芝茸 ； 太空包栽培法 ； 深層培養法

並列摘要

The research reported herein was to study diverse production methods of Ling chih (Ganoderma tsugae Murrill). In polypropylene, bag cultivation, Ling chih was suitably inoculated in March in Mid-Taiwan region, and harvested during May to November. Ling chili was harvested every 20 days and each bag produced about 75g of Ling chih and about S fruiting bodies, while baby Ling chih was harvested every 10 days and each bag produced about 101 g of baby Ling chih and about 9 fruiting bodies. With regards to the biological efficiency in fruiting body cultivation, baby Ling chih was evidently higher than Ling chih (1.05＞0.56). In shaken flask culture, the relationship of dry mycelial biomass and pH value, residual sugar content, laccase activity and polysaccharide concentration indicated that at day 12 the highest amount of dry biomass (18.74 g/L) was obtained with the highest amount polysaccharide concentration (1.38 g/L). The results of proximate composition showed that the carbohydrate, ash, crude lipid and crude protein contents of mycelia were 26.98, 5.18, 21.86 and 26.59%, respectively, significantly higher than those of fruiting bodies (both Ling chih and baby Ling chih), which were 10.41-17.17, l.69-2.62, 5.72-6.50 and 8.81-13.78%, respectively. In terms of crude fiber contents, Ling chih (73.37%) obviously more than baby Ling chih (59.93%), and in turn exceedingly higher mycelia (19.39%) and fermentation filtrate (1.58%). This research could provide valuable information for cultivation and submerged culture of native Ling chih. Also, the physiological effects of Ling chili, baby Ling chih, mycelia and fermentation filtrate could be a further area of investigation.

並列關鍵字

Ganoderma tsugae ； baby Ling chih ； polypropylene bags ； submerged culture