台灣中部犬焦蟲18S rRNA基因之序列與親緣分析

Sequence and Phylogenetic Analyses of the 18S rRNA Gene of Babesia canis in Dogs in Central Taiwan

謝玉貞(Yu-Chen Hsieh) ； 曾秋隆(Chau-Loong Tsang) ； 鍾楊聰(Yang-Tsung Chung)

《農林學報》 60卷3期 (2011/09) Pp. 209-216

https://doi.org/10.30089/JAF.201109.0003

摘要

本研究主要針對台灣中部地區自然感染焦蟲症之犬隻，利用聚合酶連鎖反應及18S rRNA基因定序方法進行病原體檢測及其分子特性之探討。首先抽取36個犬血液樣本的基因體DNA，然後分別擴增標的基因之節段、定序並與GenBank資料庫中的相關序列進行核苷酸排比。所有自10個台灣分離株擴增出來的18S rDNA序列（455個鹼基對長）全皆相同，並且與先前其他國家或地區所發表的Babesia canis vogeli序列有非常高的相似性（99.1-99.5%）。根據18S rDNA序列所做的親緣分析結果顯示，台灣分離株和先前已確認的日本株相近。這些結果首度提供台灣犬隻感染B. canis vogeli的分子證據。

關鍵字

犬焦蟲 ； 親緣關係分析 ； 定序分析 ； 分子特性

並列摘要

Canine babesiosis represents an important veterinary medical problem. The present study aimed at characterizing Babesia canis strains in naturally infected dogs in central Taiwan, using a combination of polymerase chain reaction (PCR) and sequence analysis of 18S rRNA gene. Genomic DNA was extracted from 36 canine blood samples and the target genes were separately amplified, sequenced, and aligned with corresponding sequences available in the GenBank database. All 18S rDNA sequences (455 bp) amplified from the ten Taiwanese isolates were identical to each other and had very high similarity (99.1-99.5%) with previously reported Babesia canis vogeli sequences. A phylogenetic analysis based on the 18S rDNA sequence demonstrated that the Taiwanese isolates were closely related to a strain previously identified from Japan. These results provided the first molecular evidence showing infection of dogs with B. canis vogeli from Taiwan.

並列關鍵字

Babesia canis ； Phylogenetic analysis ； Sequence analysis ； Molecular characterization