- 期刊
液化澱粉芽孢桿菌BPD1發酵配方測試
Test of Fermentation Formulae using Bacillus Amyloliquefaciens BPD1
摘要
本研究針對液化澱粉芽孢桿菌BPD1進行發酵配方試驗，初期以搖瓶進行培養試驗，發現最適配方為糖蜜0.5%、乳糖1%和大豆分離蛋白2%。以此配方進行培養條件測試，結果顯示接種24小時內菌數提升1000倍，24至48小時菌數提升10倍菌數無顯著差異。其他培養條件如培養溫度於25℃和30℃、不同pH值對菌數則皆無影響。透過10公升發酵槽的試驗，顯示以糖蜜0.5%、乳糖1%和大豆分離蛋白2%作為培養基，轉速200至300rpm、溫度30℃，可得到菌數4×10^9 CFU/mL以上之發酵液。利用1噸大型發酵槽以相同的培養基培養，發酵條件為溫度30℃、轉速160至200 rpm、通氣量0.5 vvm， 其菌數可達1.2×10^9 CFU/mL 以上；修改培養基配方後，利用1噸大型發酵槽以糖蜜3%、大豆分離蛋白1%和硫酸亞鐵0.045%培養基培養，發酵條件為溫度30℃、轉速170rpm、通氣量0.5 vvm，其菌數可達1.4×10^9 CFU/mL，此配方改善高泡問題，減少消泡劑之使用，降低成本。本試驗說明此一發酵配方可以用來大量生產液化澱粉芽孢桿菌。
關鍵字
液化澱粉芽孢桿菌、發酵、益生菌。並列摘要
This project aimed at using Bacillus amyloliquefaciens (BPD1) to conduct fermentation formula test. At the beginning, we engaged in culture test by using shake flask and found that the best formula was 0.5% molasses, 1% lactose, and 2% isolated soy protein. Then we conducted culture condition test with this formula and found that bacterial count increased 1000 times within 24 hours and then 10 times between 24-48 hours, which showed no significant differences. Other culture conditions such as temperature of 25℃ or 30℃ and pH value made no differences to bacterial count. Through test of 10-liter fermentation tank, BPD1 reached ≥ 4×10^9 CFU/mL by using 0.5% molasses, 1% lactose, and 2% isolated soy protein as culture medium, with stirring speed from 200 to 300 rpm and temperature of 30℃. As for 1-ton fermntation tank, by using the same culture medium, BPD1 reached ≥ 1.2 × 10^9 CFU/mL with temperature of 30℃, 160 rpm, and ventilation 0.5 vvm. After adjusting formula, BPD1 reached ≥ 1.4 × 10^9 CFU/mL by using 1-ton fermntation tank and culture medium of 3% molasses, 1% isolated soy protein, and 0.045% FeSO4, with temperature of 30 ℃, 170 rpm, and ventilation 0.5 vvm. This formula improves foaming problem, cutting down on antifoaming agent and lowering the cost. Overall, this study shows that the fermentation formula is satisfactory for mass production of BPD1.