The effect of three light sources: (white fluorescent lamps, WFL and two types of cold cathode fluorescent lamps, CCFLs) on the characteristics of in vitro grown cultures of three orchid species (Phalaenopsis, Oncidium, Dendrobium nobile) was studied. The commercial criteria of plantlets were used to evaluate the effect of the light sources. The plantlets were taken from vessels at different culture periods and their growth characteristics were measured. Fresh weight, plantlet height, leaf area and chlorophyll index but not number of leaves and roots were affected by the light sources. The CCFL 2S was adequate for Phalaenopsis and CCFL 3S had the best performance for Dendrobium nobile. Both CCFL light sources were adequate for Oncidium.