Many fungicides are being removed from the commercial market because they do not readily degrade in natural systems and/or they leave potentially hazardous residues in food and feed products. This has created a demand for safer products to prevent or delay the development of fungicide resistance or to reduce the risk of chemical pollution. In recent years, there has been considerable interest in using Bacillus subtilis (Bs), a gram-positive, spore-forming bacterium, as a biocontrol agent because of its ability to antagonize pathogens and repress plant diseases. In 2001, the first Taiwan domestic commercial biofungicide was registered for the control of pea powdery mildew. Bacillus subtilis has also been used to control soil-borne fungal pathogens under greenhouse conditions and in the field. However, there has been no report on comparisons of bioactivities among Bs-based products. We first established a bioassay method for Bs-based products on the basis of their inhibition activity against plant pathogens. Then, the bioactivities of 3 Bs-based products to 6 pathogenic fungi were individually tested. We report herein that only 1 Bs-based product was highly effective. These results show that the quality of these 3 Bs-based products is variable.