- 期刊
相思樹枝條抽出物抗氧化活性之初探
Preliminary Studies on the Antioxidant Activity of Ethanolic Extracts from the Twig of Acacia Confusa Merr.
摘要
本研究針對相思樹枝條抽出物及其二氯甲烷可溶部、正丁醇可溶部、水可溶部等各分離部進行抗氧化活性評估。抗氧化活性試驗包括DPPH自由基清除效應、超氧自由基捕捉試驗、還原力測定、金屬螯合效果及脂質過氧化抑制效果等；此外，並測定其總酚含量。試驗結果顯示，相思樹枝條抽出物之各分離部中以正丁醇可溶部效果最佳，在抑制DPPH自由基及超氧自由基之EC50分別為13.3及6.3μg/mL。至於枝條之總酚含量，仍以正丁醇可溶部的含量最多(706.9 mgGAE/g)，本試驗結果證實相思樹枝條之總酚含量與抑制超氧自由基成正相關。另外，正丁醇可溶部對金屬螯合及還原能力方面亦具最佳活性，此可溶部螯合金屬的EC50約為310μg/mL，而Quercetin及Catechin皆大於2500μg/mL。至於抑制脂質過氧化之效果，仍以正丁醇可溶部效果最佳（EC50為15.9μg/mL）。綜合上述試驗結果確知相思樹枝條具有極佳的抗氧化能力，極具開發應用之潛力。
並列摘要
The objective of this study is to assess the antioxidant activity of ethanolic extract and its soluble fractions from the twig of Acacia confusa Merr. The antioxidant activities were carried out using various antioxidant assays, including DPPH free radical and superoxide radical scavenging assays, reducing power assay, and metal chelating ability assay. In addition, total phenolic contents of these extracts were also determined. Results revealed that, among the various fractions, the butanol soluble fraction which contains the highest percentage of phenolic compounds exhibited the strongest antioxidant activity. The EC50 value of butanol soluble fraction exhibited 13.3 and 6.3 μg/mL scavenging activity on DPPH free radical and superoxide radical, respectively. In addition, the butanol soluble fraction also exhibited the highest reducing power and the metal chelating ability. Its EC50 value for metal chelating ability was 310 μg/mL, nonetheless the EC50 values of quercetin and catechin were more than 2500 μg/mL. As for the inhibition of lipid peroxidation, the butanol soluble fraction (EC50 15.9 μg/mL) also exhibited the strongest ability. These results demonstrated that the ethanolic extracts of twig of A. confusa have excellent antioxidant activities, indicating its potential applications are worthy of further investigations.
並列關鍵字
Acacia confuse ； Antioxidant activity ； Extracts ； Free radical ； Total phenolic contents ； Twig被引用紀錄
延伸閱讀
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