In this study antifungal activities of wood, twig, bark and leaf essential oils from black heartwood-type Cryptomeria japonica D. Don against four wood decay fungi and six plant pathogenic fungi were investigated. In addition, the yields of essential oils obtained by water distillation were compared and their constituents were determined by gas chromatography-mass spectroscopy (GC-MS) analyses. The yield of essential oils from four parts of C. japonica was in the decreasing order of leaf (18.85mL/kg; 1.42%)>wood (7.59mL/kg; 0.38%)>twig (2.14mL/kg; 0.05%)>bark (1.58mL/kg; 0.03%). From the results of GC-MS analyses, the wood essential oil was found to contain mainly γ-cadinene (22.07%) and δ-cadinene (12.12%), the main constituents of twig essential oil were α-eudesmol (10.87%) and δ-cadinene (10.41%), the bark essential oil has ferruginol (14.70%) and phyllocladanol (12.22%) as main compounds, and β-elemol (20.48%) and ent-kaur-16-ene (20.23%) were the dominant components of leaf essential oil. Results from antifungal tests demonstrated that wood essential oil had the strongest antifungal activity against four wood decay fungi and six plant pathogenic fungi. It was proven that the antifungal activity correlates well with the presence of α-cadinol, β-elemol and β-eudesmol.
In this study antifungal activities of wood, twig, bark and leaf essential oils from black heartwood-type Cryptomeria japonica D. Don against four wood decay fungi and six plant pathogenic fungi were investigated. In addition, the yields of essential oils obtained by water distillation were compared and their constituents were determined by gas chromatography-mass spectroscopy (GC-MS) analyses. The yield of essential oils from four parts of C. japonica was in the decreasing order of leaf (18.85mL/kg; 1.42%)>wood (7.59mL/kg; 0.38%)>twig (2.14mL/kg; 0.05%)>bark (1.58mL/kg; 0.03%). From the results of GC-MS analyses, the wood essential oil was found to contain mainly γ-cadinene (22.07%) and δ-cadinene (12.12%), the main constituents of twig essential oil were α-eudesmol (10.87%) and δ-cadinene (10.41%), the bark essential oil has ferruginol (14.70%) and phyllocladanol (12.22%) as main compounds, and β-elemol (20.48%) and ent-kaur-16-ene (20.23%) were the dominant components of leaf essential oil. Results from antifungal tests demonstrated that wood essential oil had the strongest antifungal activity against four wood decay fungi and six plant pathogenic fungi. It was proven that the antifungal activity correlates well with the presence of α-cadinol, β-elemol and β-eudesmol.