- 期刊
臺灣杉Tcmago及TcY14基因表現之差異性研究
Differential Expression of the Tcmago and TcY14 in Taiwania (Taiwania Cryptomerioides Hayata)
摘要
Mago nashi (mago)及Y14基因不僅與動物發育過程中之卵子形成、胚發生及生殖決定因子有關，並於植物發育過程調節生殖器官之形成機制上扮演了重要之角色。為了解臺灣杉Tcmago及TcY14於各種生理調節之反應，我們選殖並定義出含2490個鹼基對之Tcmago啟動子，以及含1780個鹼基對之TcY14啟動子。由啟動子分析顯示，Tcmago及TcY14的啟動子具有相似之特殊功能序列，其可被植物賀爾蒙（包括生長素、離層酸、吉貝素、水楊酸等）、環境逆境（包括光、冷、創傷、乾燥等），以及組織之專一性（種子、葉、莖、根、胚、花粉、保衛細胞）等調控。以北方轉印法分析其基因表現，Tcmago及TcY14對吲哚乙酸處理具生理反應，並隨濃度0μM到600μM，基因表現量逐漸增加。此外，藉由Tcmago及TcY14基因表現會被水楊酸及甲基茉莉酸處理所誘導之結果推測其可能參與植物之防禦機制。此結果與Tcmago及TcY14啟動子之結構具一致性。
並列摘要
Mago nashi (mago) and Y14 not only play essential roles in oogenesis, embryogenesis and germ-line determination during animal development, but also regulate reproductive organ formation during plant development. To understand the physiological functions of these two genes, a 2496 bp promoter region of Tcmago and a 1780 bp promoter region of TcY14 from Taiwania cryptomerioides were cloned and characterized. The results obtained from promoter analysis showed that both Tcmago and TcY14 promoter regions composed similar motifs and regulation patterns which were regulated by hormones (auxin, ABA, GA, SA), environmental stresses (light, cold, wounding, drought) and specific tissues (seed, leaf, shoot, root, embryo, pollen, guard cell). According to the results of northern blot analysis, both Tcmago and TcY14 responded to IAA treatment and increased gradually from 0 to 600 μM. Additionally, Tcmago and TcY14 may be involved in plant defense which was supported by the observations of their expressions induced by SA and MeJA treatment. The result was consistent with the promoter structures of Tcmago and TcY14.