不同木屑培養基對牛樟芝生長之效應

Effects of Different Sawdust Media on Growth of Antrodia cinnamomea

蔡博雅(Bo-Ya Tsai) ； 王靖茹(Jing-Ru Wang) ； 李明仁(Ming-Jen Lee)

《中華林學季刊》 45卷2期 (2012/06) Pp. 183-199

https://doi.org/10.30064/QJCF.201206.0004

摘要

牛樟芝（Antrodia cinnamomea Chang and Chou）為多孔菌科（Polyporaceae）、薄孔菌屬（Antrodia）的臺灣特有菌種，著生於牛樟（Cinnamomum kanehirae Hayata）腐朽心材的內壁。本研究旨在分離、鑑定及培養牛樟芝菌株，並將其培養於木屑培養基中，以探討培養牛樟芝菌株的最佳培養基，冀能培養出高品質的牛樟芝，避免盜伐牛樟立木，以達保育牛樟的目的。研究結果顯示，利用菌絲形態及核糖體DNA 5.8S序列鑑定所分離的菌株為牛樟芝。在木屑培養基方面，牛樟芝菌株於相思樹（Acacia confusa Merr.）木屑培養基的生長量較在樟樹（Cinnamomumcamphora (L.) Sieb）木屑培養基者佳，米糠添加量為10 % 時其生長量為最佳，添加牛樟葉粉可促進牛樟芝菌絲體的生長。

關鍵字

牛樟芝 ； 牛樟 ； 木屑培養基 ； 生長 ； 米糠 ； 相思樹 ； 樟樹

並列摘要

Antrodia cinnamomea Chang and Chou, a heart-rot wood decay fungus of stout camphor (Cinnamomum kanehirae Hayata), belonging to genus Antrodia, family Polyporaceae, is an endemic fungal species in Taiwan. In this study, strains of A. cinnamomea were isolated, identified and cultured in different sawdust media. The effects of different media on growth of strains of A. cinnamomea were investigated in order to provide more information for cultivating A. cinnamomea. The results showed that the isolated strains were identifi ed as A. cinnamomea via hyphal morphology and ribosomal DNA 5.8S sequence analysis. The results revealed that the growth of A. cinnamomea in Taiwan acacia (Acacia confusa Merr.) sawdust media was higher than that in camphor tree (Cinnamomum camphora (L.) Sieb) sawdust media. Meanwhile, addition of 10% rice bran and leave powder of stout camphor tree into the sawdust media promoted mycelia growth of strains of A. cinnamomea.

並列關鍵字

Acacia confusa ； Antrodia cinnamomea ； Cinnamomum camphora ； Cinnamomum kanehirae ； Growth ； Rice bran ； Sawdust media