臺灣產八角茴香科植物之分子親緣關係－利用ITS序列、花粉粒及形態特徵來推論

The Phenogenetic Relationship of Illiciaceae Plants in Taiwan-Determined by ITS DNA Sequence, Pollen Grains and Morphological Features

張良芳(Liang-Fang Chang) ； 何坤益(Kuen-Yih Ho) ； 呂福原(Fu-Yuan Lu)

《台灣農學會報》 12卷2期 (2011/04) Pp. 186-200

https://doi.org/10.6730/JAAT.201104_12(2).0006

摘要

本研究探討臺灣所產八角茴香科植物之親緣關係，應用其細胞核基因組內轉間隔區(internal transcribed spacer, ITS)序列分析，參考花粉粒與外觀形態特徵，結果由分子系統發育樹呈現紅花類群與白花類群之關係。紅花類群之紅花八角(I. arborescens)是臺灣地區較早出現之種類，群內之大雪山八角(I. tasueshanensis)分佈大雪山地區，與紅花八角分布重疊，但族群內株數甚少，仍未見結實之母株，因而建議處理為紅花八角之種內生態型。而白花類群之白花八角(I. philippinense var. philippinense)為臺灣產於高山較冰河期後出現種類；東亞八角(I. tashiroi)為東亞地區廣泛分佈種，因花粉粒與心皮數與白花八角明顯為不同之獨立種；大武八角(I. philippinense var. oblongum)則與東亞八角於分子親緣屬於同期發育之親緣類群，其花粉粒與外觀形態特徵無穩定變異性狀，因而建議處理為其種內生態型。

關鍵字

八角茴香科 ； 分子 ； 親緣關係 ； 內轉間隔區internal transcribed spacer, ITS

並列摘要

Based on the the taxonomic group determined by Chang (2009), we further investigated examined the phenogenetic relationship of I. arborescens, I. Philippinense, I. tashiroi and I. tasueshanensis in Illiciaceae by sequence analysis of internal transcribed spacer, (ITS) and morphylogy of the pollen grains. Phenogenetic analysis classified these species into two groups associated with flower color: the red flower color group and white color group. In red flower group, I. arborescens is the primitive species growing in Taiwan. With the overlapping growth region, I. arborescens and I. tasueshanensis are distributed in the Snowy Mountains region. However, a few plants in I. tasueshanensis populations, rarely and lacking the firaling and, may be categorized as I. arborescens. In white flower group, I. philippinense var. philippinense grows in the higher altitude and has appeared after the ice age in Taiwan. I. tashiroi is widely distributed in eastern Asia and significantly different from I. philippinense var. philippinense in pollen and carpel number. With the identical phenogenetic group and limited variation in morphological traits of the pollens, we propose that I. philippinense var. oblongum will be classified as the ecological type of I. tashiroi.

並列關鍵字

Illiciaceae ； phenogenetic relationship ； internal transcribed spacer