春蘭（＂Cymbidium goeringii＂ var. ＂goeringii＂）根莖誘導芽體與發育

Bud Induction and Development from Rhizomes Culture of ＂Cymbidium goeringii＂ var. ＂goeringii＂

廖成康(Cherng-Kang Liao) ； 宋明德(Ming-De Sung)

《台灣農學會報》 16卷2期 (2015/06) Pp. 129-140

https://doi.org/10.6730/JAAT.201506_16(2).0003

摘要

本研究主要建立春蘭（＂Cymbidium goeringii＂ var. ＂goeringii＂）根莖培養以誘導芽體形成並正常發育的組織培養方法。為探討根莖部位對誘導芽體形成之影響，將無菌播種衍生的根莖，取根莖頂端長約0.5 cm，或只取中間段約1 cm及2 cm，分別培養於添加0, 0.1, 0.5, 1.0, 2.0mgL^(-1) N-phenyl-N'-1,2,3-thidiazol-5-ylurea（TDZ）的1/2 MS液體培養基，培養5週，調查根莖誘導芽體分化的比率。TDZ顯著促進每根莖芽體形成數，未添加TDZ的培養基僅根莖的生長並無芽體的分化。將不同根莖部位衍生的單芽，移至含有0.1, 0.5, 1.0 mg L^(-1) indole-3-butyric acid（IBA）及1.0 g L^(-1)花寶一號的MS固體培養基培養6週，IBA對小苗形成率並無顯著的影響，以0.5 mg L^(-1) TDZ誘導根莖分化的芽體，其小苗形成率顯著降低。將0.25 mg L^(-1) TDZ誘導的單芽，培養於3 g L^(-1)花寶一號之1/2 MS固體培養基，培養6週後，有93.8%的芽體可形成完整植株，可提供商業大量生產之需。

關鍵字

春蘭 ； 大量繁殖 ； 根莖

並列摘要

The purpose of this study was to establish a protocol for mass production of ＂Cymbidium goeringii＂ var. ＂goeringii＂ by tissue culture. The rhizomes derived from seeds were divided into a tip segment (0.5 cm) and non-tip segment (1 and 2 cm), and then they were cultured on 1/2 MS liquid medium with 0, 0.1, 0.5, 1.0 or 2.0 mg L^(-1) TDZ for 5 weeks. The percentage of rhizome differentiation was examined. The results showed the number of buds per rhizome could be increased by TDZ. The single bud derived from rhizome was cultured on 1/2 MS medium with different concentrations of IBA and HYPONeX No.1. The frequency of plant regeneration was increased to 93.8% in 3 g L^(-1) HYPONeX No.1 containing medium if the single bud was originally induced from 0.25 mg L^(-1) TDZ cultured rhizome.

並列關鍵字

＂Cymbidium goeringii＂ var. ＂goeringiia＂ ； mass propagation ； rhizome