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Introduction: Brucella endocarditis is a rare but severe complication of brucellosis. Although it is observed in less than 2% of the brucellosis cases, it is the main cause responsible for up to 80% of infection-related deaths in brucellosis. Case Report: Herein, we present a case of Brucella endocarditis that developed on a native aortic valve. The diagnosis was proven by positive blood cultures and isolation of Brucella melitensis from the excised valve. Conclusion: Although endocarditis associated with brucella infection is rare, it may be more frequently observed in the regions where brucellosis is endemic. Therefore, especially in these regions, Brucella endocarditis must be considered during the diagnosis of infective endocarditis.

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