The effects of excess calorie intake on nitrogen (N) balance were studied with five young men of 18 to 20 years of age. Protein intake was fixed at 0.51g/kg/day and 87% of the total N was supplied with mixture of milk and egg (1:1) in a liquid formula. The daily activity of the subjects was set at moderate lever and, therefore, the basal diet provided 46 kcal/kg of energy as recommended by the FAO/WHO Expert Committee. The calorie intake was varied from 46 to 51, 56, 58.5 and 61kcal/kg in randomized order. The excess calorie was supplied by mung bean starch and fat at equal proportion. Test diets were given to the subjects for 10 days during the first period, successively followed by 7-day periods with rotating test diets. The mean daily N balance increased progressively towards positive side from -18.5mg/kg to -7.4, -2.2, -2.1 and +4.7mg/kg as energy intake was increased. It was apparent that there was significant positive correlationship between the energy intake and the N balance (r=+0.97, p<0.01). It is not certain from the present study, whether a higher than 61kcal/kg energy supply would further increase the N retention at the same protein intake level. However, it is conceivable that the ”Protein sparing effect” of carbohydrate and fat is at least near the maximum at 61kcal/kg level. The correlationship between the energy intake and urinary N was significantly negative (r=-0.985, p<0.01). It vas noticed, however, that when the energy intake was increased to above 56kcal/kg levels, the fall in urinary N became smaller. The mean apparent and true protein digestibilities of the test diets were 78.6 and 96.2% respectively.
The effects of excess calorie intake on nitrogen (N) balance were studied with five young men of 18 to 20 years of age. Protein intake was fixed at 0.51g/kg/day and 87% of the total N was supplied with mixture of milk and egg (1:1) in a liquid formula. The daily activity of the subjects was set at moderate lever and, therefore, the basal diet provided 46 kcal/kg of energy as recommended by the FAO/WHO Expert Committee. The calorie intake was varied from 46 to 51, 56, 58.5 and 61kcal/kg in randomized order. The excess calorie was supplied by mung bean starch and fat at equal proportion. Test diets were given to the subjects for 10 days during the first period, successively followed by 7-day periods with rotating test diets. The mean daily N balance increased progressively towards positive side from -18.5mg/kg to -7.4, -2.2, -2.1 and +4.7mg/kg as energy intake was increased. It was apparent that there was significant positive correlationship between the energy intake and the N balance (r=+0.97, p<0.01). It is not certain from the present study, whether a higher than 61kcal/kg energy supply would further increase the N retention at the same protein intake level. However, it is conceivable that the ”Protein sparing effect” of carbohydrate and fat is at least near the maximum at 61kcal/kg level. The correlationship between the energy intake and urinary N was significantly negative (r=-0.985, p<0.01). It vas noticed, however, that when the energy intake was increased to above 56kcal/kg levels, the fall in urinary N became smaller. The mean apparent and true protein digestibilities of the test diets were 78.6 and 96.2% respectively.