限制給食對青春發育期至成熟期之雄鼠細胞發育及功能之影響

Restriction of Energy Intake in Relation to Cellular Development and Function of Male Rats from Puberty to Maturation

劉麗雲(Li-Yun Liu) ； 蔡玉吉(Yuh-Chi Tsai)

《中華民國營養學會雜誌》 7卷1&2期 (1982/09) Pp. 59-74

https://doi.org/10.6691/JCNS.198209_7(1_2).0005

摘要

本實驗係將限制給食時間由青春發育期開始，直至成熟期，俾進一步探討動物於邁入該生命階段受給食限制亦即營養素攝取量不足時的營養不良現象，對器官功能發展可能產生的影響，實驗結果顯示如下：
1．限制給食之食物能量達自由給食者之30 ~ 40%時，可維持供試雄鼠之恆定體重。
2．限制給食用以維持恆定體重所需之熱能，在時間年齡(Chronological age)之愈早期愈高。
3．在體重年齡(Body weight age)不足之情形下，生理年齡(physiological age)乃受時間年齡之影響。
4．限制給食維持恒定體重之雄鼠，由於腦部在早期已發育成熟，故最不受影響，而脾臟、肝臟、心臟、腎臟則依序受到影響。
5．以Protein/DNA所表示之單位細胞大小，顯示出限制給食維持恒定體重之雄鼠，除腦部組織外，其餘器官如心臟、肝臟、脾臟、腎臟等組織皆顯示出萎縮現象。
6．以RNA/DNA所表示之單位細胞蛋白質合成能力，可顯示出除腦部外，其餘各組織器官均受限制給食影響而有蛋白質合成能力降低之現象。
7．各器官之細胞分化速率及增大期因受限制給食影響而有降低與延後現象，是以導致成熟期亦相對延後。
8．供試雄鼠在維持恆定體重之條件下限制給食，因限制能量之給與，血液除白血球含量較低外，其餘之成份如血紅素、紅血球、血漿蛋白質含量等並無明顯差異。
9．測定血漿(plasma)之G. O. T.及G. P. T.活性雖顯示出在限制給食之條件下略有增加，然由其遞增之數值觀之，其肝臟之功能並無受到顯著影響。

並列摘要

This study is going to disclose the influence of restricted food intake on the cellular as well as functional development of growing rat during the period from puberty to maturation. The results revealed that:
1. The male rats could maintain constant weight provided calories of restricted feeding are 30-40% as those of feeding ad libitum.
2. In restricted feeding, the calories needed to maintain constant weight are higher at the younger chronological age.
3. Even the body weight of test rat was maintained constant, the development of physiological age was still affected by chronological age.
4. Restricted feeding had no significant influence on the rat brain development, on the other hand, the development of spleen, liver, heart, kidney was affected orderly.
5. Using Protein/DNA ratio as a parameter for cell size, exception brain tissue, all the organs tested, liver, heart, kidney and spleen, showed cellular atrophy.
6. The RNA/DNA ratio (the protein synthetic ability of unit cell) of the most organs except brain decreased by the dietary energy restriction.
7. The rate of cellular division and their augmentation of test organs as well as maturation have delayed by the restriction of dietary energy intake.
8. No significant difference for the blood erythrocytes, hematocrit, hemoglobin and total plasma protein was found between the ad libitum feeding and the restricted feeding groups.
9. Under the energy restriction the plasma G. O. T. and G. P. T. activity increased, but non-significantly.

並列關鍵字

restricted feeding ； malnutrition ； metabolic body size ； puberty ； physiological age ； chronological age