天然膳食纖維與生技合成難消化性多醣類對部分腸道細菌産酸之影響

Effects of Natural Dietary Fiber and Biosynthetic Polysaccharides on the pH Reduction by Selected Intestinal Microflora

楊雅清(Ya-Ching Yang) ； 蔣鳳琳(Fong-Lin Tsiang) ； 　丘志威(Chih-wei P. Chiu) ； 蔡敬民(Ching-Min E.Tsai)

《中華民國營養學會雜誌》 18卷3&4期 (1993/10) Pp. 165-174

https://doi.org/10.6691/JCNS.199310_18(3_4).0003

摘要

本實驗旨在探討腸道細菌利用天然膳食纖維與生物技術合成多醣類之産酸情形。實驗選用人體腸道常見的六株細菌，分別是Bifidobacterium bifidum 、Bifidobacterium longum、Lactobacillus acidophilus、Enterococcus faecalis 、Bacteroides fragilis 、Escherichia coli, 利用體外試驗之方法，於37℃、厭氧的情況之下，培養於含有纖維素、關華豆膠、阿拉伯膠、聚糊精、泛纖維的五種不同難消化性醣類的培養液中。在培養0，4，8，12，24，48小時從檢測培養液pH値變化情形以作爲評定對醣類利用之指標。結果B.bifidum和B. longum對泛纖維與聚糊精之利用産酸較天然膳食纖維強，而泛纖維之産酸情形又較聚糊精強。L. acidophilus、E. faecalis和 E. Coli 亦會利用泛纖維與聚糊精，而使pH値下降，但下降幅度比B. bifidum或B. longum均明顯地較小；而這三株菌利用天然膳食纖維之pH値下降情則不明顯。B. fragilis對於所有天然膳食纖維與生技合成難消化性多醣類之利用産酸情形均不佳。

關鍵字

膳食纖維 ； 合成多醣類 ； 腸道細菌

並列摘要

This study was to investigate the effect of natural dietary fibers and biosynthetic polysaccharides on the acid production by selected intestinal microflora. Six bacteria strains were cultured anaerobically in broth containing five different indigestible dietary fibers namely, cellulose guar gum, gum arabic, polydextrose and pine fiber at 37℃. Acid production was examined at 0, 4, 8,12, 24, 48 hours. The results showed that Bifidobacterium bifidum and Bifidobacterium longum in biosynthetic polysaccharides had more acid production than that in the natural dietary fibers. Moreover, pine fiber had lower pH than polydextrose. Lactobacillus acidophilus, Enterococcus faecalis and Escherichia coli could utilize pine fiber and polydextrose to produce acids, but the pH values of these three bacteria were higher than the ones of B.bifidum and B. longum. The difference of these three bacteria strains in utilizing the natural dietary fibers to reduce the pH values was not significant. The pH reduction effect by Bacteroides fragilis was not observed under the fiber sources.

並列關鍵字

Dietary fiber ； Synthetic oligosaccharides ； Intestinal microflora