補骨脂水萃取物及補骨脂酚對大白鼠初代肝細胞之抗氧化保護作用

Antioxidative Protection of Water Extract of Psoralea Fruit and Bakuchiol on Primary Rat Hepatocytes

葉佳聖(Jia-Sheng Yeh) ； 沈立言(Lee-Yan Sheen) ； 李宗貴(Chong-Kuei Lii) ； 蔡順仁(Shun-Jen Tsai)

《中華民國營養學會雜誌》 22卷3期 (1997/08) Pp. 303-324

https://doi.org/10.6691/JCNS.199708_22(3).0005

摘要

Protective effect of water extract of psoralea fruit and bakuchiol on the viability, lipid peroxidation, glutathione (GSH) levels and activities of GSH-related enzymes of primary rat hepatocytes under oxidative stress induced by glucose/glucose oxidase was investigated. Hepatocytes were incubated with or without water extract of psoralea fruit (0.6mg/mL) or bakuchiol (0.125mM), and 50μg/mL glucose, 100μg/mL glucose oxidase except for the blank. The results reveal that the lactate dehydrogenase leakage of hepatocytes incubated with water extract of psoralea fruit or bakuchiol was significantly suppressed; showing only about half that of the control at 8 hr incubation, and the protective effects lasted till 48 hr (p＜0.05). As for the lipid peroxidation of hepatocytes, water extract of psoralea fruit or bakuchiol significantly (p＜0.05) controlled the lipid peroxidation induced by glucose and glucose oxidase. On the other hand, water extract of psoralea fruit or bakuchiol helped maintain GSH and oxidized glutathione levels in hepatocytes under oxidative stress, and even induced more GSH production. Regarding the aspect of the GSH-related enzymes activities, water extract of psoralea fruit or bakuchiol stopped the activities of glutathione peroxidase, glutathione S-transferase and glutathione reductase decrease what caused by oxidative stress and even to elevate the activities of them. Thus, water extract of psoralea fruit and bakuchiol protected hepatocytes from oxidation damage via increasing the GSH levels and elevating the activities of GSH-related enzymes of hepatocytes.

關鍵字

補骨脂 ； 補骨脂酚 ； 氧化緊迫 ； 麩胱甘肽

並列摘要

Protective effect of water extract of psoralea fruit and bakuchiol on the viability, lipid peroxidation, glutathione (GSH) levels and activities of GSH-related enzymes of primary rat hepatocytes under oxidative stress induced by glucose/glucose oxidase was investigated. Hepatocytes were incubated with or without water extract of psoralea fruit (0.6mg/mL) or bakuchiol (0.125mM), and 50μg/mL glucose, 100μg/mL glucose oxidase except for the blank. The results reveal that the lactate dehydrogenase leakage of hepatocytes incubated with water extract of psoralea fruit or bakuchiol was significantly suppressed; showing only about half that of the control at 8 hr incubation, and the protective effects lasted till 48 hr (p＜0.05). As for the lipid peroxidation of hepatocytes, water extract of psoralea fruit or bakuchiol significantly (p＜0.05) controlled the lipid peroxidation induced by glucose and glucose oxidase. On the other hand, water extract of psoralea fruit or bakuchiol helped maintain GSH and oxidized glutathione levels in hepatocytes under oxidative stress, and even induced more GSH production. Regarding the aspect of the GSH-related enzymes activities, water extract of psoralea fruit or bakuchiol stopped the activities of glutathione peroxidase, glutathione S-transferase and glutathione reductase decrease what caused by oxidative stress and even to elevate the activities of them. Thus, water extract of psoralea fruit and bakuchiol protected hepatocytes from oxidation damage via increasing the GSH levels and elevating the activities of GSH-related enzymes of hepatocytes.

並列關鍵字

psoralea fruit ； bakuchiol ； oxidative stress ； glutathione