飲食膽固醇與禁食對大白鼠脂質代謝的影響

Effect of Dietary Cholesterol and Fasting on Lipid Metabolism in Rats

盧義發(Yi-Fa Lu) ； 林宗美(Chung-May Lin)

《中華民國營養學會雜誌》 23卷4期 (1998/11) Pp. 335-345

https://doi.org/10.6691/JCNS.199811_23(4).0002

摘要

本研究之目的在探討飲食中膽固醇的添加及24小時的禁食對大白鼠脂質代謝的影響。28隻六週大之雄性Wistar大白鼠分成四組，分別為不含膽固醇之餵食組與禁食組、添加l%膽固醇之餵食組與禁食組，餵食四週，禁食組於犧牲前禁食24小時。分析項目為血清及肝臟各種脂質含量與各脂肪酸組成。結果顯示，飲食中膽固醇的添加並不影響血清總膽固醇和三酸甘油酯的含量，而24小時的禁食則顯著降低血清中三酸甘油酯的量；另外，膽固醇的添加與禁食皆會降低血清磷脂質含量。在肝臟脂質方面，膽固醇與禁食顯著增加膽固醇的含量，而三酸甘油酯的量則受飲食中膽固醇的添加及禁食此二因子的交互作用影響。在脂肪酸組成方面，飲食中膽固醇添加皆會降低血清及肝臟中18:0及20:4(n-6)的相對含量，增加18:1(n-9)及l8:2(n-6)的相對含量；而24小時禁食則顯著增加血清及肝臟中18:0及20:4(n-6)，降低18:1(n-9)的相對含量。這些結果顯示膽固醇與禁食兩者對脂質代謝具有交互作用存在。

關鍵字

膽固醇 ； 禁食 ； 脂質 ； 大白鼠

並列摘要

The purpose of this study was to investigate the effect of dietary cholesterol and fasting on lipid metabolism in rats. Twenty-eight Wistar rats aged 6 weeks were divided into 4 groups, fed diets with (NC, normal diet) 1% cholesterol or without 1% cholesterol (N, normal diet) for 4 weeks. At the termination of feeding, two groups of rats were fasted 24 hours (fasted groups) and the others were freely fed (fed groups). Serum and liver lipid content and tissue fatty acid composition were determined. Serum total cholesterol and triacylglycerols were not significantly different between N and NC groups. However, the concentration of serum triacylglycerols in fasted groups was markedly lower than that of fed groups. Liver triacylglycerols were affected by dietary cholesterol, fasting and their interaction. NC groups caused the decrease of 18:0 and 20:4 (n-6) levels, but with increased 18:1 (n-9) and 18:2 (n-6) contents in serum and liver. Fasting increased 18:0 and 20:4 (n-6) levels, and decreased the relative content of 18:1 (n-9) fatty acid in serum and liver. The results suggest that there appeared to be an interaction of dietary cholesterol with fasting with respect to lipid metabolism.

並列關鍵字

cholesterol ； fasting ； lipids ； rats