Older than 1-year Taiwania (Taiwania cryptomerioids Hay.) seedlings, grown in polyethylene bag at Ho-Sueh nursery on National Taiwan University Experimental Forest, were subjected to pruning 1/3 of lower branches. The morphological parameters of seedlings and the accumulation and partitioning of assimilate after treatment were determined to comprehend the response of seedling to pruning. The results showed that the growth of height, collar diameter, and dry weight of shoot system were promoted by pruning. The chlorophyll content and accumulation of starch and soluble sugar in both shoot and root system were increased. However, the response of root dry weight was negative. Nevertheless, the combination of branch-pruning with other nursery practices for Taiwania Seedling were valuable to further study.