利用電泳薄膜分析法作血清中胸腺淋巴球激素之定量分析

An Electrophoretic Quantitative Determination of Lymphocyte Stimulating Hormone in Human Serum

汪叔游(S. Y. Wang) ； 黃正一(C. Y. Huang) ； 陳李仲惠(C. H. Chern Ly)

《Journal of Medical Sciences》 2卷1期 (1977/01) Pp. 443-451

摘要

本實驗係以Robey氏所精製之胸腺蛋白結晶品作為電泳薄膜上分離點定性之認定，再由動物胸腺之提取物中與人類血清提取物中之分離點與之比較，以其Rf之恒定性作為認定之標準，從而測定50位我國空軍軍官血清中胸腺淋巴球激素之含量，其平均濃度為2mg/ml其範圍從0.3到5.5mg/ml不等，7位小兒科病患年齡從4歲到7歲，其平均含量為1.1mg/ml，範圍從0.3到2.5mg/ml，如此把以往只能利用柱層色彩分析法測定之胸腺淋巴球激素改到在普通醫院的檢驗室中就可測定，為臨床檢驗提供一簡易而準確性可靠的胸腺淋巴球激素之定量及定性方法。

並列摘要

The purpose of this work is to provide a simple, accurate quantitative assay for Lymphocyte Stimulating Hormone (LSH) by using the routine clinical microzone electrophoresis method. This new method has two keystone: one is using Dr. Robey's pure extraction of LSH crystal as an identified reference, the other is using chymotrypsinogen standard curve to determine the LSH quantitative concentration of separation point on membrane. we obtain the same Rf value from the extraction of animal thymus gland and that of human serum as Dr. Robey's product. This assay provides a simple rapid precise and high sensitive routine method for studying LSH protein.