自製血液細菌培義瓶之評價

An Evaluation of Homemade Blood Culture Bottles for Routine Blood Culture

沈弘德(H. D. Shen) ； 朱夢麟(M. L. Chu) ； 李堃杰(K. J. Lee) ； 丁雪青(S. C. Ting) ； 趙德讓(T. J. Chao)

《Journal of Medical Sciences》 4卷3期 (1982/01) Pp. 1315-1322

摘要

血液細菌培養是臨床細菌室重要的工作項目之一。自行製作血液細菌培養瓶，可節省大量經費。但因自製者未抽眞空，未灌入多數細菌生長所需之CO2或N2,H2及CO2之混合氣體，產品仍多缺失。所以本實驗比較本院自製血液瓶與市售之國內、外血液瓶之檢出效率及其汚染之可能性，供作推廣自製之參考。經將市售血液瓶於接種檢體後，置於一般37℃培養溫箱培養；自製血瓶則於接種檢體後鬆蓋置於含5%CO2及可充入混合氣之厭氧培養溫箱（Glove box）中培養，在65組的實驗中，我們發現自製血瓶之細菌分離培養效率，並不低於市售之血瓶，且製作成本低廉。使用自製血瓶，發現生長需求較複雜之細菌，如H. influenzae之分離率偏低，為了觀察是否係自製血瓶之培養基與培養情況不適於此種細菌之生長？所以取不同稀釋度之H. influenzae type b菌液，分別接種於市售之國內、外血瓶與自製血液細菌培養瓶中。經將國內、外市售血瓶及部份自製血瓶緊蓋置於一般37℃培養溫箱中，部份自製血瓶則鬆蓋後分別置於CO2培養溫箱或Glove box中培養，結果顯示H. influenzae type b在上述不同血液培養瓶及培養情況下，均生長良好。

並列摘要

Sixty-five sets of blood culture samples were compared to evaluate the sensitivity of bacteria growing in different sources of blood culture bottles. Experimental results showed that blood culture bottles made in our laboratory were as sensitive as those perchased from commerical sources for routine blood culture.For those bacteria requiring more fastidious nutrients and culture conditions for growth, such as H. influenzae type b, blood culture bottles made in our laboratory were also shown to be effective.