血清中免疫複合物的測定：C1q微板酵素結合免疫吸附法

Detection of Circulating Immune Complexes by a C1q-Microplate ELISA System

張木蘭(Mu-Lan Chang) ； 周昌德(Chung-Tei Chou) ； 李錦發(Ching-Fa Lee) ； 李明仙(Ming-Hsien Liu) ； 趙有誠(Jou-Chen Chao) ； 張德明(Deh-Ming Chang)

《Journal of Medical Sciences》 6卷3期 (1986/03) Pp. 231-236

摘要

A sensitive and simple procedure for detection and quantitation of circulating immune complexes (CIC) in human sera has been developed by utilizing Clq-microplate ELISA system.The utilization of an enzyme marker reagents, as opposed to 125 I-labled reagents is extremely advantageous, since it is safe, stable, economical, and fast.We applied this system to detect CIC in sera of 30 normal healthy controls and 83 patients with various diseases, including 23 cases of systemic lupus erythematosus (SLE), 25 cases of rtheumatoid arthritis (RA), 19 cases of malignant tumor (MT) (pulmonary carcinoma in 10 and hepatoma in 9) and 16 cases of acute type B hepatitis (HB). The average level of CIC (ug AHG eq/ml) in normal control group and four different patient groups were as follows: 12.7±6.12 for normal group, 29.54±15.24 for RA, 67.93±42.22 for SLE, 41.66±24.6 for MT and 27.34±17.79 for HB. The average of all the four different patient groups was significantly higher than that of the normal control group (P＜0.001). Our results were some what better or equivalent to that by other methods in the literature. The conclusion that C1q microplate ELISA system is a safe, simple, economical and sensitive method for detecting circulating immune complexes is thus made.

關鍵字

補體微量酵素免疫複合物測定法

並列摘要

A sensitive and simple procedure for detection and quantitation of circulating immune complexes (CIC) in human sera has been developed by utilizing Clq-microplate ELISA system.The utilization of an enzyme marker reagents, as opposed to 125 I-labled reagents is extremely advantageous, since it is safe, stable, economical, and fast.We applied this system to detect CIC in sera of 30 normal healthy controls and 83 patients with various diseases, including 23 cases of systemic lupus erythematosus (SLE), 25 cases of rtheumatoid arthritis (RA), 19 cases of malignant tumor (MT) (pulmonary carcinoma in 10 and hepatoma in 9) and 16 cases of acute type B hepatitis (HB). The average level of CIC (ug AHG eq/ml) in normal control group and four different patient groups were as follows: 12.7±6.12 for normal group, 29.54±15.24 for RA, 67.93±42.22 for SLE, 41.66±24.6 for MT and 27.34±17.79 for HB. The average of all the four different patient groups was significantly higher than that of the normal control group (P＜0.001). Our results were some what better or equivalent to that by other methods in the literature. The conclusion that C1q microplate ELISA system is a safe, simple, economical and sensitive method for detecting circulating immune complexes is thus made.

並列關鍵字

C1q-micro ELISA-circulating immune complexes