The degradation kinetics of isoamyl nitrite were studied under the conditions of various material of containers, temperatures, buffers, and the addition of stabilizers. Determining by the validated HPLC analytical method showed that the degradation kinetics of isoamyl nitrite followed the apparent zero order reaction model. The rate constants of reaction were calculated. The activation energy of the degradation of isoamyl nitrite in ethyl alcohol was about 10 kcal/mole. The presence of magnesium oxide decreased the degration rate of amyl nitrite constant significantly.