微透析技術之簡介與應用

Introduction and Application of Mecrodialysis

劉亞平(Yia-Ping Liu) ； 童吉士(Che-Se Tung)

《Journal of Medical Sciences》 14卷2期 (1993/10) Pp. 71-80

摘要

微透析技術是近年來被廣泛地應用在生命科學各類研究中的新寵。它是一種進步的化學取樣方式，利用物質通透、擴散、均質化等原理，藉著單向循流將取樣區域的化學物質攜出，繼之交由高效液相層析儀進行測定。由於微透析技術可以有效地反應出細胞外液中多神經傳遞物的動態變化，亦能在微觀下進一步瞭解各種分子及藥物的作用情形。因之對研究神經藥精神科學有極大的幫助。本文就徵透析技術的演進、理論及機轉做一介紹，涵蓋細胞生理、神經傳遞物、以及測定通等層面。並就本實驗室在應用時的實際經驗進行討論。以期拋磚引玉。

關鍵字

微透析 ； 回復率 ； 胞泄作用 ； 膠樣變性 ； 神經傳遞物

並列摘要

Microdialysis, an improved technique developed from push-pull cannula method, can collect multiple chemical substances within microenvironmental space. The microdialysis probe, like push-pull probe, was designed to be a one-way infusion tubing system, but the perfusate was contained inside a semi-permeable membrane located at the tip of the probe. The dialysate passed the tube and carried the extracellular fluid of the target region, then, dialysate could be separated by high performance liquid chromatography on revere phase columns and estimated by electrochemical detection. It has been applied to the in vivo study of the release and metabolism of various neurotransmitters, and also opened up a wealth of possibilities to monitor chemical events within the brain and to study the working mechanism of various drugs. In this article, we introduced the development and mechanism of microdialysis, including cellular physiology, neurotransmission, and the analysis technique; we also discussed our experience in the previous study.

並列關鍵字

Microdialysis ； recovery rate ； exocytosis ； gliosis ； neurotransmitters