- 期刊
- OpenAccess
對乳癌有關抗原具專一性知單株抗體的發展現況
MONOCLONAL ANTIBODIES SPECIFIC TO BREASTCANCER-ASSOCIATED ANTIGENS
摘要
歐美許多國家中，乳癌（breast cancer）的死亡率一直是婦女癌症的第一位。我國乳癌的發生率是婦癌的第二位，而死亡率是婦癌的第四位；其發生率與死亡率並有逐年提升的情形，因此有關乳癌診斷及治療的研究，在臨床上具有重大之意義。抗乳癌單株抗體在乳癌的診斷及治療上深具潛力；目前學者已應用乳癌細胞膜粹取物（B72.3）、乳癌細胞株（MBRl）及雌性素受體（D547 Spr, D75P3r）等為免疫原製成單株抗體；但這些抗體目前還無法直接用來診斷乳癌。而單株抗體B-14乃本學院孟慶樑教授，以乳癌細胞株BC-M1的細胞膜粹取蛋白為免疫原所製備的單株抗體。本實驗室以此單株抗體B-14於乳癌的組織中，偵測到一分子量約為62kd的蛋白質；由於該抗原含有甘露糖（mannose）、唾液酸（sialic acid）及N-乙醯胺基葡萄糖（N-acetyl-glucosamine）等單醣類，因此命名為GP-62。目前正探討其臨床上的可能應用。
並列摘要
Breast carcinoma is one of major malignancy among the female in many countries Monoclonal antibodies specific to breast tumor-associated antigens are invaluable for early diagnosis and follow-up. Many monoclonal antibodies had been generated for this purpose with various antigens, e.g. membrane extracts of metastatic breast carcinoma (B72.3), breast carcinoma cell line (MBR1) or estrogen receptor (D547 Spr and D75P3r). The application of these antibodies in clinical diagnosis are being evaluated, and the roles of these antigens in the carcinogenesis of breast carcinoma are being investigated. A monoclonal antibody, B-14 is generated through the cell fusion of myeloma cells with spleen cells immunized with BCM -1, a breast carcinoma cell line. Immunoblots of BCM-1 cell extracts revealed a glycol protein of 62 Kd.
延伸閱讀
- Tai, T. W. (2008). 乳癌專一性單株抗體的製備與功能之探討 [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2008.10441
- 陳柏志（2007）。南台灣女性乳癌病人其人類白血球組織抗原-DQA1, -DQB1等位基因多型性分析之相關性研究〔碩士論文，高雄醫學大學〕。華藝線上圖書館。https://doi.org/10.6832/KMU.2007.00091
- 陳國庭（2007）。錸(I)-188三羰基標誌Trastuzumab單株抗體作為乳癌造影及治療劑之研究〔碩士論文，國立清華大學〕。華藝線上圖書館。https://doi.org/10.6843/NTHU.2007.00104
- Yang, C. Y. (2020). 乳癌進程中胞外體之蛋白質體分析及機制探討 [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU202100159
- 陳柄梅、林嘉伯（1994）。Application of Monoclonal Antibodies for the Study of Tumor Associated Antigens。藥物食品檢驗局調查研究年報，()，132-139。https://doi.org/10.6946/ASRNL.199409.0132