超氧歧化酶之毛細管電泳分析法

本文介紹一種可行的牛紅血球中銅鋅－超氧歧化酶之毛細管電泳定量分析法。此法使用pH8.7之0.02M硼酸鹽緩衝液。其標準曲線之線性範園為12.5-200 μg/ml（r=0.999）。其回收率為99.96至107.6%之間。而同日標準差與異日標準差分別為1.36%與2.90%（n=3）。本毛細管電泳法可應用於超氧歧化酶之安定性分析研究上。

關鍵字

超氧歧化酶；毛細管電泳分析；安定性研究分析法

並列摘要

An efficient capillary electrophoretic method was developed for the assay of superoxide dismutase in bovine erythrocyte. The buffer solutions used in this method was of 0.02M sodium borate (pH8.7). The linear range was 25-200 μg/mL (r=0.999) and recovery was 99.96 to 107.6%. The relative standard deviations for intraday and intereday analyses were 1.36% and 2.90% (n=3) respectively. This capillary electrophoretic method could be applied as a stability indicating assay method of bovine erythrocyte superoxide dismutase.

並列關鍵字

bovine erythrocyte superoxide dismutase ； capillary electrophoresis ； stability indicating assay method

延伸閱讀

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蕭懿民（1998）。毛細管電泳在農藥主成份分析之應用。藥毒所專題報導，(50)，1-7。https://doi.org/10.29952/YTSCTPT.199807.0001
徐婉玲（2008）。以毛細管電泳研究唾液酸酶對不同鍵結唾液酸之反應動力學〔碩士論文，國立臺北科技大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0006-2107200817202700
Lin, C. C. (2011). High-throughput enzyme-linked immunosorbent assay (ELISA) for the determination of protein tyrosine sulfation [master's thesis, National Chiao Tung University]. Airiti Library. https://doi.org/10.6842/NCTU.2011.00675
Ken, C. F., Chen, H. T., Chang, R. C., & Lin, C. T. (2008). Biochemical Characterization of a Catalase from Antrodia Camphorata: Expression in Escherichia coli and Enzyme Properties. Botanical Studies, 49(2), 119-125. https://www.airitilibrary.com/Article/Detail?DocID=1817406X-200804-49-2-119-125-a

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超氧歧化酶之毛細管電泳分析法

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