The Development of a Cold Adapted Influenza Virus A/Taiwan/1/30/86(H1N1)

一株適低溫生長之流行性感冒病毒influenza A/Taiwan/1/30/86（H1N1）（A/Taiwan/30），可在29℃表現生長能力但無法在37℃生長，已在我們研究室以連續降溫的重覆接種培養法被建立成功。此病毒株具有適低溫生長的表現穩定且對小白鼠的毒力減低的特性。當此適低溫病毒懸液被稀釋至3.2×10^(-6) HAU/ml且被接種（每枚雞胚胎接種0.1ml之病毒懸液）到10天之雞胚胎尿囊腔後，可偵測到病毒的生長的只有那些在29℃培養之胚胎，而在37℃培養者不生長。另一方面，我們亦發現各種不同種類之動物血清中含有抑制influenza A/Taiwan/1/86（H1N1）對紅血球的凝集作用，但這些有抑制凝集作用的物質（HISs）可被接受器破壞酵素（RDEs）所破壞。此結果顯示，這些HISs的化學特性及其外形結構可能與紅血球表面上之接受器相類似，可被RDEs所辨識和破壞。

關鍵字

流行性感冒病毒 A/Taiwan/1/86（H1N1）；適低溫病毒株；血球凝集抑制試驗

並列摘要

The influenza A/Taiwan/1/30/86(H1N1) (A/Taiwan/30) cold- adapted virus, expressing the ability to reproduce at 29℃ and inability to reproduce at 37℃ (the ca phenotype), was successfully established by repeated passages at progressively lower temperatures in our laboratory. This strain is phenotypically stable and attenuated for mice. When the cold-adapted virus suspension was diluted to 3.2 10^(-6) HAU/ml and inoculated (0.1 ml of the virus suspension per chicken embryo) into the allantoic cavity of 10-day old chicken embryos, virus replication can only be detected in those cultures incubated at 29℃ but not at 37℃ On the other hand, we found that sera from various animal species somehow contained hemagglutination inhibition substances (HISs), exhibiting an inhibitory effect on the hemagglutination of influenza virus A/Taiwan/1/86(H1N1), which could be destroyed by the receptor destroying enzymes (RDEs). This result suggests that the chemical nature and the conformational structure of these HISs may be similar to those hemagglutinin receptors on the permissive erythrocytes and can be recognized and destroyed by the RDEs.

並列關鍵字

Influenza A/Taiwan/1/86/ (H1N1) ； Cold-adapted strain ； Hemagglutination inhibition

國際替代計量

The Development of a Cold Adapted Influenza Virus A/Taiwan/1/30/86(H1N1)

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