Regulation of Glucose Transporter 1 by IL-1β Stimulation in Rat Articular Chondrocytes

Background: The facilitative glucose transporters (GLUTs) are essential for chondrocyte survival and production of glycosaminoglycan. The objective of this study was to examine the glucose transporter 1 (GLUT1) expression in rat articular chondrocytes and its regulation in response to IL-1βstimulation. Methods: Primary cultures of normal articular chondrocytes were isolated from rat knee joints. GLUT1 expression and regulation in the presence or absence of IL-1β was evaluated by reverse transcription-polymerase chain reaction (RT-PCR), western blotting, and immunocytochemistry. SN50 peptide was used to inhibit nuclear factor-kappa B (NF-kB) activation. Results: IL-1β stimulation upregulated GLUT1 mRNA and protein levels in a time dependent manner. The gene upregulation and protein expression of GLUT1 induced by IL-1β were suppressed in the presence of SN50 (P＜0.05, in gene expression). Conclusion: GLUT1 upregulation may be associated with NF-kB activation, in rat articular chondrocytes following IL-1β stimulation. Whether the expression of GLUT1 is related with the pathogenesis of osteoarthritis merits further investigation.

並列關鍵字

Glucose transporter ； Chondrocyte ； IL-1β ； Nuclear factor-kB

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