Characterization of Monoclonal Antibodies against Dengue Serotype 2

Yu-Yine Huang ； Li-Jeng Tarn ； Hsueh-Li Chou ； Szu-Chia Lai

《Journal of Medical Sciences》 33卷5期 (2013/10) Pp. 255-261

https://doi.org/10.6136/JMS.2013.33(5).255

並列摘要

Background: Dengue virus (DEN), the pathogen behind dengue hemorrhagic fever, remains a public health problem in Asia and South America. Method: Monoclonal antibodies (MAbs) specifically against dengue serotype 2 were generated by fusion of immunized mouse spleen cells with NS1/1-Ab4-1 myeloma cells. DEN-2 strain PL046, a local strain isolated from Taiwanese dengue fever patients, was used in the immunization. Results: The specificities of MAbs were determined by Western blot and ELISA assays. Six anti-E and twenty-one anti-NS1 MAbs were generated from mice immunized with PL046 strain, two of the six anti-E MAbs strongly inhibited infection by PL046 and 16681 strains. Ten of the twenty-one anti-NS1 MAbs were specifically against dengue serotype 2. Overall, three anti-E and twelve anti-NS1 MAbs were produced and characterized. The isotypes were also determined and described. Conclusions: The MAbs generated in this study may provide a powerful tool for antigenic analysis and clinical diagnosis.

並列關鍵字

dengue ； monoclonal antibodies (MAbs)

參考文獻

Falconar AK, Young PR. Immunoaffi nity purifi cation of native dimer forms of the fl avivirus non-structural glycoprotein, NS1. J Virol Methods 1990;30:323- 332.

Winkler G, Maxwell SE, Ruemmler C, Stollar V. Newly synthesized dengue-2 virus nonstructural protein NS1 is a soluble protein but becomes partially hydrophobic and membrane-associated after dimerization. Virology 1989;171:302-305.

Crooks AJ, Lee JM, Easterbrook LM, Timofeev AV, Stephenson JR. The NS1 protein of tick-borne encephalitis virus forms multimeric species upon secretion from the host cell. J Gen Virol 1994;75:3453- 3460.

Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flamand M. Enzyme-linked immunosorbent assay specifi c to Dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol 2002;40:376-381.

Koraka P, Burghoorn-Maas CP, Falconar A, Setiati TE, Djamiatun K, Groen J, Osterhaus AD. Detection of immune-complex-dissociated nonstructural-1 antigen in patients with acute dengue virus infections. J Clin Microbiol 2003;41:4154-4159.