- 期刊
- OpenAccess
MicroRNA-125a Expression in Isolated Lymphocytes and Decreased Regulated on Activation, Normal T‑cell Expressed and Secreted Production during Cardiac Surgery with Cardiopulmonary Bypass
摘要
Background: Cardiopulmonary bypass (CPB) induces postoperative immunosuppression, including decreased T-cells and lower plasma regulated on activation, normal T-cell expressed and secreted (RANTES) concentrations. MicroRNA-125a negatively regulates RANTES expression in activated T-cells. The aims were to investigate microRNA-125a expression in T-cells and RANTES production following CPB. Materials and Methods: Twenty-eight patients undergoing elective cardiac surgery were included in this study. Arterial blood was sampled at six sequential points (before anesthesia induction, before CPB, at 2, 4, 6, and 24 h after beginning CPB) for plasma RANTES concentrations by enzyme-linked immunosorbent assay. T-lymphocytes were isolated from whole blood at four points (before anesthesia, before CPB, at 2 and 4 h after beginning CPB) for intracellularmicroRNA-125a expression by quantitative real-time reverse transcription polymerase chain reaction in 14 patients. Perioperative laboratory data and variables were also recorded. Results: The plasma RANTES concentrations decreased significantly at 2-24 h after beginning CPB, with concurrent reduction of postoperative lymphocyte counts, as compared with the preanesthesia level (P ＜ 0.001). Intra-T-cell microRNA-125a expression was activated at 2 and 4 h, however, without significance (P = 0.078 and 0.124, respectively). The plasma RANTES levels at 4 h were not correlated with CPB time (P = 0.671), anesthesia time (P = 0.305), postoperative extubation time (P = 0.508), and Intensive Care Unit (ICU) stay (P = 0.756). Three patients expired with pneumonia- or mediastinitis-related septic shock in the ICU. Conclusion: Plasma RANTES concentrations were depressed till 24 h following CPB, with reduced lymphocytes after cardiac surgery. MicroRNA-125a expression in T-lymphocytes was not correlated with perioperative variables and its role in downregulation of RANTES production needs to be determined.
延伸閱讀
- Xiao, G., Tang, H., Wei, W., Li, J., Ji, L., & Ge, J. (2014). Aberrant Expression of MicroRNA-15a and MicroRNA-16 Synergistically Associates with Tumor Progression and Prognosis in Patients with Colorectal Cancer. Gastroenterology Research and Practice, 2014(), 302-309-042. https://doi.org/10.1155/2014/364549
- Hung, Y. C. (2017). Mechanism of action of a disintegrin, trimucrin in suppressing LPS-induced activation of phagocytes andattenuating myocardial ischemia-reperfusion injury [doctoral dissertation, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU201704512
- Lin, T. C., Lin, F. Y., Lin, Y. W., Hsu, C. H., Huang, G. S., Wu, Z. F., Tsai, Y. T., Lin, C. Y., Li, C. Y., & Tsai, C. S. (2015). Matrix Metalloproteinase-9 Production following Cardiopulmonary Bypass Was Not Associated with Pulmonary Dysfunction after Cardiac Surgery. Mediators of Inflammation, 2015(), 352-356-132. https://doi.org/10.1155/2015/341740
- 劉璿慧（2020）。Analysis of Cardiovascular Disease-Associated MicroRNA by Nucleic Acid Amplification Technology in Conjunction with Capillary Electrophoresis〔碩士論文，國立暨南國際大學〕。華藝線上圖書館。https://doi.org/10.6837/ncnu202000270
- Akker, F. V. D., Jager, S. C. A. D., & Sluijter, J. P. G. (2013). Mesenchymal Stem Cell Therapy for Cardiac Inflammation: Immunomodulatory Properties and the Influence of Toll-Like Receptors. Mediators of Inflammation, 2013(), 371-383-033. https://doi.org/10.1155/2013/181020