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Protective Effect of Tongxinluo on Oxidative Injury Induced by Angiotensin Ⅱ in Rats
並列摘要
Purpose: To investigate the effect of Tongxinluo (TXL), a traditional Chinese herbal medicine, on aortic biological variation after angiotensin Ⅱ (Ang Ⅱ)-induced vascular oxidative injury.Methods: Randomly, 50 Sprague-Dawley (SD) rats were divided into three groups: sham, Ang Ⅱ, and Ang Ⅱ+TXL. A pump with Ang Ⅱ was embedded in the rat backs in the Ang Ⅱ and Ang Ⅱ+TXL groups, whereas a pump containing physiological saline was emplaced in the sham group. TXL was delivered through a gastric tube in the Ang Ⅱ+TXL group. Endothelin-1 (ET-1), tumor necrosis factor-α (TNF-α) and nitric oxide (NO) in the plasma were examined 14 days later; the aortic endothelial cells were observed under a scanning electron microscope (SEM); the expressions of endothelial nitric oxide synthase (eNOS), nuclear factor-кB (NF-кB), and vascular cell adhesion molecule-1. (VCAM-l) were measured via immunohistochemistry assays; apoptotic cells were investigated with DeadEnd(superscript TM) colorimetric TUNEL System; and the NAD(P)H oxidase subunit P22phox mRNA was examined through reverse transcription polymerase chain reaction.Results: The plasma concentrations of ET-l and TNF-α increased significantly in the Ang Ⅱ group in comparison with the sham one, but decreased in the Ang Ⅱ+TXL group. The same observations occurred regarding NF-кB, VCAM-l, apoptosis and P22phox mRNA expressions in the aortic tissues. However, eNOS expression in the aorta produced a reverse response, which was ameliorated significantly by TXL.Conclusion: The findings suggested that TXL could play a potential role in inhibiting the oxidative injury induced by Ang Ⅱ by reducing the inflammation and the apoptotic process via the P22phox pathway.
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