Chondrogenesis in vitro and in vivo using human mesenchymal stem cells cultured in alginate beads

Mesenchyaml stem cells (MSCs) from bone marrow that are culture-adher-ent have been reported to contain osteoprogenitor cells, chondrogenitor cells, and stem cells of adipogenic and fibroblastic cell lineages. Various biomaterials in which cells can be embedded and delivered in a controlled manner have been studied. Alginate forms a viscous solution when dissolved in 0.9% saline and gels on contact with divalent cations. The viability and phenotype maintenance of chondrocytes in alginate beads have been well documented. However, little is known about the effects of microencapsulation in alginate on chondrogenesis of human MSCs. In this study, human MSCs encapsulated in alginate beads were cultured in vitro with a defined medium, consisting of serum-free DMEM-LG with ITS-Premix, TGFβ1 (10 ng/ml), dexamethasone (10-7M), and ascorbate 2-phosphate (50μg/ml). MSCs in alginate assumed a round morphology with lacunae around them, and reverse transcription-polymerase chain reaction (RT-PCR) revealed the expression of both COL2A1 and COL10A1 after 1 week in culture. MSCs-alginate beads were then transplanted under the dorsal skin of 4-6 week-old nude mice (nu/nu). The constructs were easily visualized and easily dissected from surrounding subcutaneous tissue at 8 weeks after transplantation. The gross appearance of the specimens, opaque and yellowish in color, hard and elastic in texture, resembled that of cartilage. Histological findings agreed with the clinical determination of hyaline cartilage, characterized by isolated cells the clinical determination of hyaline cartilage, characterized by isolated cells with basophilic ground substance at the periphery of cells.

並列關鍵字

Alginate beads ； In vitro ； In vivo ； Mesenchymal stem cells(MSCs) ； Chondrogenesis ； Biomaterials

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