This study was undertaken to evaluate the results of two-stage treatment of late chronic total knee arthroplasty (TKA) infection using an antibiotic-impregnated cement spacer. Twenty-nine knees in 29 patients with late chronic TKA infection were treated with debridement, component removal, insertion of an antibiotic-impregnated cement spacer with Steinmann pin fixation, and second-stage revision TKA. All patients followed individualized regimens of intravenous antibiotic therapy for 2 weeks and oral antibiotic therapy for at least 4 weeks. Follow-up averaged 72 months (range, 31-118 months). The average range of motion after second-stage surgery was 107° (range, 85°-135°), and the average Hospital for Special Surgery knee score was 89.4 (range, 74-93). The average visual analogue scale pain score was significantly improved. Two patients died after second-stage surgery owing to end-stage renal disease under hemodialysis. One patient had a recurrence of infection. The success rate was therefore 96.3% (26 of 27 patients). Use of a static antibiotic-impregnated cement spacer can eradicate chronic late TKA infection, facilitate second-stage surgery, reduce postoperative pain, improve function, and preserve maximum range of motion after second-stage surgery.