精胺酸與醯胺麩胺酸對感染抗甲基青黴素金黃色葡萄球菌小白鼠的影響

Effect of Arginine and Glutamine on the Mice Infected with Methicillin-Resistant Staphylococcus Aureus

王銘富(Ming-Fu Wang) ； 黃鏽萍(Hsiu-Ping Huang) ； 鍾惠美(Hei-Mei Chung) ； 吳雪華(Shie-Hwa Wu)

《中華民國營養學會雜誌》 22卷4期 (1997/11) Pp. 425-434

https://doi.org/10.6691/JCNS.199711_22(4).0006

摘要

The object of this study was to investigate the effect of arginine and glutamine supplements on BALB/c mice infected with methicillin resistant Staphylococcus aureus (MRSA). The mice were randomly divided into 4 groups. Each group of mice was fed as follows: 20% casein diet (Control Group A), 20% casein diet supplemented with 2% arginine (Group B), 20% casein diet supplemented with 4% glutamine (Group C) and 20% casein diet supplemented with 2% arginine and 4% glutamine (Group D). After 10 days of feeding, each mouse was challenged with MRSA organisms intravenously. Twenty days later, the survival rate was recorded and pathological examinations were performed. The colony forming units (CFU) in the kidney and spleen of most of the surviving mice were determined. The result showed that A, B, C and D groups survival rates were 20%, 60%, 66% and 80%, respectively. For the CFU of kidney and spleen, groups B, C and D were lower than A, but had increased (p＜0.05) spleen weight. The conclusion is that a diet supplemented with arginine or glutamine would enhance resistance to the mice infected with MRSA.

關鍵字

精胺酸 ； 醯胺麩胺酸 ； 抗甲基青黴素金黃色葡萄球菌 ； 小白鼠

並列摘要

The object of this study was to investigate the effect of arginine and glutamine supplements on BALB/c mice infected with methicillin resistant Staphylococcus aureus (MRSA). The mice were randomly divided into 4 groups. Each group of mice was fed as follows: 20% casein diet (Control Group A), 20% casein diet supplemented with 2% arginine (Group B), 20% casein diet supplemented with 4% glutamine (Group C) and 20% casein diet supplemented with 2% arginine and 4% glutamine (Group D). After 10 days of feeding, each mouse was challenged with MRSA organisms intravenously. Twenty days later, the survival rate was recorded and pathological examinations were performed. The colony forming units (CFU) in the kidney and spleen of most of the surviving mice were determined. The result showed that A, B, C and D groups survival rates were 20%, 60%, 66% and 80%, respectively. For the CFU of kidney and spleen, groups B, C and D were lower than A, but had increased (p＜0.05) spleen weight. The conclusion is that a diet supplemented with arginine or glutamine would enhance resistance to the mice infected with MRSA.

並列關鍵字

arginine ； glutamine ； methicillin resistant Staphylococcus aureus ； mice