- 期刊
- OpenAccess
銳葉楊梅萃取物誘導子宮頸癌細胞凋亡
Extract of Myrica rubra var. acuminata Induced Apoptosis in a Cervical Carcinoma Cell Line
摘要
子宮頸癌為婦女最常發生的癌症之一，儘管目前在醫學的診斷或治療上的技術已經十分進步，子宮頸癌的發生率及致死率仍然居高不下。本研究採集台灣產之藥用植物，以FeCl3試劑檢測其多酚類之含量，挑檢出十四種富多酚類之植物，分別對口腔癌細胞株(KB)及子宮頸癌細胞株(HeLa)進行毒性試驗篩選。結果顯示：除黃槿、檉柳、紅淡比外其餘十一種萃取物在濃度100 μg/mL皆對腫瘤細胞具有超過50%之細胞生長抑制率，其中又以銳葉楊梅萃取物對正常胃纖維母細胞(NGF)毒性較小。故進一步探討其抑制HeLa生長之作，發現對HeLa細胞毒性具濃度及時間依存性。根據觀察其細胞核內染色質濃縮之形態變化，及DNA斷鏈之測量亦顯示銳葉楊梅萃取物誘導HeLa凋亡。然而，100 μg/mL之銳葉楊梅萃取物對子宮頸纖維母細胞(NCF)有細胞毒性，且觀察其對NCF之形態影響，亦顯示細胞核內染色質濃縮誘導NCF凋亡，但濃度低於100 μg/mL則細胞毒性不明顯。
並列摘要
Cervical carcinoma is one of the most common cancers in women worldwide. Despite advances in diagnosis and therapy, morbidity and mortality from this malignancy are still high. In this study, 14 kinds of medicinal plants were collected from Taiwan, and the abundance of polyphenols was confirmed based on the formation of colored complexes with FeCl3. Moreover, the cytotoxic effects of test complexes were evaluated in human oral epidermal (KB) and cervical (HeLa) carcinoma cell lines as compared with the effects on normal gastric and cervical fibroblasts using the MTT assay. The results showed that extracts of Hibiscus tiliaceus, Tamarix juniperina, and Cleyera joponica had no significantly cytotoxic effects on carcinoma cell lines, but all of other extracts did at 100 μg/mL for 48 h. However, the extract of Myrica rubra var. acuminata (Mra) exerted stronger cytotoxic effects on HeLa cells and lower cytotoxicity to primary cultured normal gastric fibroblasts. The chromatin condensation effect of Mra-treated normal cervical fibroblasts was observed by light microscopy, and the mechanism of Mra-induced cytotoxic effects on HeLa cells was explored. Mra-induced cytotoxicity occurred in a dose-and time-dependent manner. HeLa cells treated with Mra exhibited chromatin condensation and DNS fragmentation as observed by microscopy, flow cytometry, and agarose gel electrophoresis, The above results suggest that Mra induced apoptosis in HeLa cells and normal cervical fibroblasts.