添加菊糖於稀釋發酵乳對人體腸道菌相之影響

Effect of Inulin Containing Fermented Milk Administration on Human Intestinal Bacterial Flora

沈祐成(You-Cheng Shen) ； 柯慧君(Hui-Chun Ko) ； 黃筠茜(Yun-Chien Huang) ； 陳肅霖(Su-Lin Chen) ； 王進崑(Chin-Kun Wang)

《臺灣營養學會雜誌》 35卷2期 (2010/06) Pp. 77-86

https://doi.org/10.6691/NSJ.201006_35(2).0004

摘要

近年來各種發酵乳產品在市場上佔有率日益增加。主要是因為發酵乳產品具有整腸的功用。本試驗目的在了解添加菊糖之稀釋發酵乳在改善腸道菌相之功效，共徵求48位18~27歲健康之受試者，隨機分成三組。本試驗進行期間，供應受試者符合國民均衡飲食建議量之飲食。本試驗共計七週，第一週為飲食調整期，第二週起每天給予受試者2瓶稀釋發酵乳或安慰劑持續4週，追蹤期2週。在試驗進行中，每2週收集一次糞便檢體進行德厭氧菌、雙歧桿菌(Bifidobacterium spp.)、乳酸桿菌(Lactobacillus spp.)、大腸桿菌(E. coli)和產氣莢膜梭菌(Clostridium perfringens)等腸道主要益生菌和有害菌的菌數測定。結果發現，受試者不論飲用有無添加菊糖之稀釋發酵乳後，均能有效地增加糞便中益生菌－雙歧悍菌佔腸道內總菌數之比值，並使有害菌大腸桿菌和產氣莢膜梭菌佔腸道內總菌數之比值減少，尤其以添加菊糖之稀釋發酵乳的效果可以維持至停止飲用後兩週。故知每日飲用2瓶稀釋發酵乳並持續4週後，的確可改善人體腸道之菌相。

關鍵字

稀釋發酵乳 ； 菊糖 ； 雙歧桿菌 ； 大腸桿菌 ； 產氣夾膜菌 ； 益生菌

並列摘要

This study is to understand whether ingestion of inulin containing fermented milk would alter the human intestinal bacterial flora. The human clinical trial employed randomized, placebo controlled, double blind design. 48 healthy participants with aged 18 to 27 years were randomly divided into three groups. The daily meals were supplied during the 7 weeks of experimental period, including an initial one week without fermented milk (run-in period), 4 weeks with two bottles (330 mL each ) per day of fermented milk, inulin containing fermented milk, and placebo, respectively (administration period), and 2 weeks follow-op period. The total aerobic bacteria, Bifidobacterium spp., Lactobacillus spp., E. coil, and Clostridiurn pertfringens of each fresh stool sample were analyzed. Results showed the Bifidobacterium/total anerobic becteria in stool was significantly increased (p＜0.05) whereas the harmful E. coil/total anerobic becteria and Clostridiurn pertfringens/total anerobic becteria were decreased after ingesting the fermented milk and inulin containing fermented milk for 4 weeks, even during the follow-up period when supplement consumption was discontinued, but no significant difference was found for placebo. In conclusion, ingestion of fermented milk or inulin containing fermented milk for 4 weeks is beneficial in improving the intestinal function by increasing the number of stool Bifidobacteria and suppressed E. coli and Clostridiurn pertfringens.

並列關鍵字

Fermented milk ； inulin ； Bifidobacteria ； E-coll Clostridium perfringens ； Probiotic