視網酸和β-胡蘿蔔素單獨或合併使用維生素E對LPS誘發的發炎反應的影響：體外研究

Effects of Retinoic Acid and β-Carotene Alone or in Combination with α-Tocopherol on LPS-Induced Inflammation: An in Vitro Study

陳珮瑩(Pei-Ying Chen) ； 鄭妃吟(Fei-Yin Jheng) ； 葉姝蘭(Shu-Lan Yeh)

《臺灣營養學會雜誌》 37卷1期 (2012/03) Pp. 1-13

https://doi.org/10.6691/NSJ.201203_37(1).0001

摘要

本研究的目的要比較2μM視網酸（RA）及β-胡蘿蔔素（BC）單獨或合併10μM維生素E 作用對RAW264.7細胞受lipopolysaccharide（LPS）誘發後，分泌促發炎激素及抑制發炎激素和活性氧（ROS）產生的情形。另外，亦比較其條件培養基對C3H10T1/2細胞的毒性。RA單獨存在培養基中顯著降低LPS誘發的TNF-α及ROS產生，但對IL-6及IL-10分泌沒有顯著影響，而合併維生素E後，所有促發炎物質均顯著降低，另外亦顯著增加IL-10的生成。BC單獨或合併維生素E對LPS所誘發的發炎調節物質表現的影響與RA有相似的趨勢，不過效果可能較差。此外，RA而非BC顯著降低條件培養基對C3H10T1/2細胞DNA的傷害，維生素E亦相加性的增加RA及BC的效果。維生素E的存在有降低RAW264.7細胞BC或RA損耗的趨勢。而2,6-di-tert-butyl-4-methylphenol（BHT；β-carotene-15,15'-dioxygenase抑制劑）與BC共同存在下，細胞內BC含量增加，而RA的量減少。BHT同時抑制BC促進IL-10分泌的效果，且其條件培養液對C3H10T1/2細胞DNA傷害亦無保護性。綜合以上，本研究結果顯示，2μM RA的抗發炎效果較同濃度BC好，維生素E的存在，可能增加二者調控發炎反應的能力。

關鍵字

β-胡蘿蔔素 ； 視網酸 ； RAW264.7細胞 ； 抑制發炎

並列摘要

The aim of this study was to compare the effects of 2 μM retinoic acid (RA) and β-carotene (BC) alone or in combination with 10 μM α-tocopherol on the secretion of pro- and anti-inflammatory cytokines as well as the production of reactive oxygen species (ROS) in RAW264.7 cells induced by lipopolysaccharide (LPS). In addition, we compared the toxicity of conditioned media toward C3H10T1/2 cells. RA alone significantly decreased the secretion of tumor necrosis factor (TNF) -α and intracellular ROS levels in RAW264.7 cells induced by LPS, but had no effect on the secretion of interleukin (IL) -6 or IL-10. RA in combination with BC significantly decreased the secretion of these proinflammatory mediators induced by LPS, and significantly increased the secretion of IL-10. The effects of BC alone or in combination with α-tocopherol on the secretion of inflammatory modulators induced by LPS were similar to or lower than those of RA. In addition, RA but not BC significantly reduced the DNA toxicity of conditioned medium toward C3H10T1/2 cells. α-Tocopherol also additionally enhanced the suppressive effect of RA or BC against DNA damage induced by conditioned medium. The existence of α-tocopherol in RAW264.7 cells tended to decrease the depletion of BC or RA induced by LPS. The presence of 2,6-di-tert-butyl-4-methylphenol(BHT)increased the level of BC, while it decreased the level of RA in RAW264.7 cells. However, BHT tended to decrease the anti-inflammatory effect of BC. In conclusion, our results suggested that the overall effects of 2 μM RA alone or in combination with α-tocopherol on anti-inflammation were better than those of 2 μM BC alone or in combination withα-tocopherol. α-Tocopherol increased the anti-inflammatory effects of RA and BC.

並列關鍵字

β-carotene ； retinoic acid ； RAW264.7 cells ； anti-inflammation