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七層塔或白鳳菜水萃物可降低以Oxonate誘導高尿酸小鼠之血漿尿酸濃度
Hypouricemic Effects of the Water Extracts of Ocimum gratissimum and Gynura formosana in Oxonate-Treated Mice
摘要
高尿酸血症的盛行率逐漸增加，近來研究更指出高尿酸血症之發生除了與痛風有關之外，更與肥胖、心臟病、糖尿病、腦血管疾病等慢性疾病皆有密切相關。本研究欲探討白鳳菜及七層塔水萃物對於高尿酸誘導小鼠之影響。白鳳菜及七層塔水萃物所測得之總多酚含量、總抗氧化能力以七層塔較高，總類黃酮含量則是兩者相當。高尿酸血症動物模式為採用ICR雄性小鼠連續7天腹腔注射氧嗪酸鉀(Potassium oxonate, PO) 250 mg/kg BW/d 以誘導高尿酸血症。動物共合成七組Vehicle組(正常控制組，注射管餵生理食鹽水)、PO組(尿酸誘導組)、AL組(Allopurinol藥物治療組) 、GF1 與GF5 組(白鳳菜水萃物100 mg/kg BW/d、500 mg/kg BW/d)、OG1與OG5 組(七層塔水萃物100 mg/kg BW/d 、500 mg/kg BW/d)，每日管餵次，連續7天。血漿尿酸濃度以PO組顯著最高，給予藥物治療之AL組則顯著下降62.9%﹒在試驗樣品組中無論是GF1、GF5 或OG1、OG5 組其血漿尿酸濃度都顯著低放PO 組(ρ＜0.05) ，尿酸抑制率平均的分別達49.2%及45.7%，但沒有明顯的劑量效應。肝臟黃嘌呤氧化酶活性於各組間雖無顯著差異，然而黃嘌呤去氫酶活性則顯著以PO組最高，AL組則顯著降低黃嘌呤去氫酶活性達32%；七層塔與白鳳菜萃出物亦可達到抑制黃嘌呤去氫酶活性的20-28%左右的抑制率。腎臟的Uric acid salt transporter protein (URAT1) 及organic anion transporter (OAT1) 兩蛋白質表現量於各組之間未見有顯著差異。本實驗結果指出，連續管餵白鳳菜與七層塔水萃物可顯著降低高尿酸誘導小鼠的血漿尿酸濃度，其降尿酸機制應與肝臟黃嘌呤去氫酶活性之抑制有關。
並列摘要
Hyperuricemia, is considered a key risk factor for gout and has also been linked to renal dysfunction, cardiovascular diseases, hypertension, hyperlipidemia, cancer, diabetes and metabolic syndrome. Therefore, controlling serum uric acid levels is widely considered to be an important issue in the prevention and treatment of these diseases. In this study, we investigated the hypouricemic effects of the water extracts of Gynura formosana and Ocimum gratissimum in mice treated with potassium- oxonate (PO). Methods: The hypouricemic effects were evaluated in ICR male mice. Animals were divided into seven groups, and all animals except the vehicle group (normal control group) were injected intraperitoneally with PO at a dose of 250 mg/kg BW/d for 7 days to induce hyperuricemia. Animals were orally administered the following test samples: saline (vehicle and PO groups). allopurinol (AL)(10 mg/kg BW/d, as the therapy group), the water extract of G. fonnosana at 100 or 500 mg/kg BW/d (the GF1 or GF5 group; reepectnvelvl, or the water extract of O. gratissimum at 100 or 500 mg/kg BW/d (OG1 or OG5 group; respecttively) for 7 consecutive days. Results: The water extracts of G. fonnosana and O. gratissimum significantly reduced plasma uric acid and also showed an inhibition effect on hepatic xanthine dehydrogenase (XDH) activity, but not xanthine oxidase (XO), compared to the PO group. Protein levels of the renal uric acid salt transporter protein (URAT1) and organic anion transporter (OAT1) also showed no significant difference among all groups. Conclusion: The hypouricemic effect of orally administered water extracts of G. formosana and O. gratissimum may mostly be due to their inhibition of hepatic XDH activity in mice treated with PO.