Micro dissection is used to obtain a particular kind of a single cell or cells from tissue sections such as cell oncogenes, tumor suppressor gens and enzymes. The purpose of this study is to establish a system based on piezoelectric ultrasonic micro-tools and motion platform. Using ultrasonic vibrations, the micro-cut knife results in easy tissue penetration. Mechanical vision assists in monitoring the tool positioning and elucidates the feasibility of cutting. To determine the optimal parameters for dissection, the vibration amplitude and frequency of the cutting tool were studied. Resistance force due to micro dissection is reduced thereafter. Finally, a low cost device for micro dissection in clinical practice is achieved.